Surgery
Volume 145, Issue 5 , Pages 558-567 , May 2009

Acute propranolol infusion stimulates protein synthesis in rabbit skin wound

  • Xiao-Jun Zhang, MD

      Affiliations

    • Shriners Hospital for Children, Galveston, TX
    • Department of Surgery, University of Texas Medical Branch, Galveston, TX
    • Corresponding Author InformationReprint requests: Xiao-Jun Zhang, MD, Shriners Hospital for Children, 815 Market Street, Galveston, TX 77550.
    • ,
  • Chengyue Meng, MD

      Affiliations

    • Shriners Hospital for Children, Galveston, TX
    • Department of Surgery, University of Texas Medical Branch, Galveston, TX
    • ,
  • David L. Chinkes, PhD

      Affiliations

    • Shriners Hospital for Children, Galveston, TX
    • Department of Surgery, University of Texas Medical Branch, Galveston, TX
    • ,
  • Celeste C. Finnerty, PhD

      Affiliations

    • Shriners Hospital for Children, Galveston, TX
    • Department of Surgery, University of Texas Medical Branch, Galveston, TX
    • ,
  • Asle Aarsland, MD, PhD

      Affiliations

    • Shriners Hospital for Children, Galveston, TX
    • Department of Anesthesiology, University of Texas Medical Branch, Galveston, TX
    • ,
  • Marc G. Jeschke, MD, PhD

      Affiliations

    • Shriners Hospital for Children, Galveston, TX
    • Department of Surgery, University of Texas Medical Branch, Galveston, TX
    • ,
  • David N. Herndon, MD

      Affiliations

    • Shriners Hospital for Children, Galveston, TX
    • Department of Surgery, University of Texas Medical Branch, Galveston, TX

,Accepted 16 January 2009.

References 

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  4. Baron PW, Barrow RE, Piere EJ, Herndon DN. Prolonged use of propranolol safely decreases cardiac work in burned children. J Burn Care Rehabil. 1997;18:223–227
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  9. Slominski A, Wortsman J. Neuroendocrinology of the skin. Endocr Rev. 2000;21:457–487
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  12. Zhang X-J, Sakurai Y, Wolfe RR. An animal model for measurement of protein metabolism in the skin. Surgery. 1996;119:326–332
  13. Zhang X-J, Chinkes DL, Wolfe RR. The flow phase of wound metabolism is characterized by stimulated protein synthesis rather than cell proliferation. J Surg Res. 2006;135:61–67
  14. Zhang X-J, Chinkes DL, Herndon DN. Folate stimulation of wound DNA synthesis. J Surg Res. 2008;147:15–22
  15. Zhang X-J, Chinkes DL, Wu Z, Martini WZ, Wolfe RR. Fractional synthesis rates of DNA and protein in rabbit skin are not correlated. J Nutr. 2004;134:2401–2406
  16. Patterson BW, Zhang X-J, Chen Y, Klein S, Wolfe RR. Measurement of very low stable isotope enrichment by gas chromatography/mass spectrometry: application to measurement of muscle protein synthesis. Metabolism. 1997;46:943–948
  17. Wolfe RR, Chinkes DL. Isotopic tracers in metabolic research: principle and practice of kinetic analysis. 2nd ed.. New York: Wiley-Liss; 2004;
  18. Phillips SM, Tipton KD, Aarsland A, Wolf SE, Wolfe RR. Mixed muscle protein synthesis and breakdown after resistance exercise in humans. Am J Physiol. 1997;273:E99–107
  19. Zhang X-J, Chinkes DL, Sakurai Y, Wolfe RR. An isotopic method for measurement of muscle protein fractional breakdown rate in vivo. Am J Physiol. 1996;270:E759–E767
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  21. Rosenblatt J, Chinkes DL, Wolfe MH, Wolfe RR. Stable isotope tracer analysis by GC-MS, including quantification of isotopomer effects. Am J Physiol. 1992;263:E584–E596
  22. Volpi E, Jeschke MG, Herndon DN, Wolfe RR. Measurement of skin protein breakdown in a rat model. Am J Physiol. 2000;279:E900–E906
  23. Singer AJ, Clark RAF. Mechanisms of disease: cutaneous wound healing. N Eng J Med. 1999;341:738–746
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  25. Gore DC, Honeycutt D, Jahoor F, Barrow RE, Wolfe RR, Herndon DN. Propranolol diminishes extremity blood flow in burned patients. Ann Surg. 1991;213:568–573
  26. Martini WZ, Chinkes DL, Wolfe RR. Quantification of DNA synthesis from different pathways in cultured human fibroblasts and myocytes. Metabolism. 2004;53:128–133
  27. Reyes-Salcido V, Villalobo-Molina R. Evidence that dl-propranolol increases thymidine kinase activity, cell mitosis, and β-adrenoceptors during rat liver regeneration. Arch Med Res. 2003;34:273–275
  28. Rixon RH, Whitfield JF. The possible cyclic AMP-dependence of an early prereplicative event that determines mitosis in regenerating rat liver. J Cell Physiol. 1973;124:157–164
  29. Hong J-H, Hwang ES, Lee CH, Lee YH, Lee SK. DL-propranolol negatively regulates the transcription of proliferating cell nuclear antigen (PCNA)-gene and thereby suppresses DNA synthesis in regenerating rat liver. Biochem Mol Biol Int. 1997;42:103–112
  30. Pullar CE, Grahn JC, Liu W, Isseroff RR. 2-Adrenergic receptor activation delays wound healing. FASEB J. 2006;20:76–86

 Supported by Grants 8600 and 8490 from the Shriners Hospital.

PII: S0039-6060(09)00066-X

doi: 10.1016/j.surg.2009.01.006

Surgery
Volume 145, Issue 5 , Pages 558-567 , May 2009

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