Surgery
Volume 146, Issue 2 , Pages 190-197 , August 2009

Mesenchymal stem cells enhance the viability and proliferation of human fetal intestinal epithelial cells following hypoxic injury via paracrine mechanisms

  • Brent R. Weil, MD

      Affiliations

    • Department of Surgery, Indiana University School of Medicine, Indianapolis, IN
    • ,
  • Troy A. Markel, MD

      Affiliations

    • Department of Surgery, Indiana University School of Medicine, Indianapolis, IN
    • ,
  • Jeremy L. Herrmann, MD

      Affiliations

    • Department of Surgery, Indiana University School of Medicine, Indianapolis, IN
    • ,
  • Aaron M. Abarbanell, MD

      Affiliations

    • Department of Surgery, Indiana University School of Medicine, Indianapolis, IN
    • ,
  • Daniel R. Meldrum, MD

      Affiliations

    • Clarian Cardiovascular Surgery, Indiana University School of Medicine, Indianapolis, IN
    • Department of Surgery, Indiana University School of Medicine, Indianapolis, IN
    • Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN
    • Center for Immunobiology, Indiana University School of Medicine, Indianapolis, IN
    • Corresponding Author InformationReprint requests: Daniel R. Meldrum, MD, 635 Barnhill Drive, Van Nuys Medical Science Building, Room 2017, Indianapolis, IN 46202.

,Accepted 2 March 2009.

References 

  1. Markel TA, Crisostomo PR, Wairiuko GM, Pitcher J, Tsai BM, Meldrum DR. Cytokines in necrotizing enterocolitis. Shock. 2006;25:329–337
  2. Ford HR. Mechanism of nitric oxide-mediated intestinal barrier failure: insight into the pathogenesis of necrotizing enterocolitis. J Pediatr Surg. 2006;41:294–299
  3. Meldrum DR. Tumor necrosis factor in the heart. Am J Physiol. 1998;274:R577–R595
  4. Wang SF, Che XM, Chen JC, Lu SY, Fan L, Wang R, et al. Treatment of short gut syndrome with early living related small bowel transplantation. Transplant Proc. 2005;37:4461–4463
  5. Khalil PN, Weiler V, Nelson PJ, Khalil MN, Moosmann S, Mutschler WE, et al. Nonmyeloablative stem cell therapy enhances microcirculation and tissue regeneration in murine inflammatory bowel disease. Gastroenterology. 2007;132:944–954
  6. Nagy RD, Tsai BM, Wang M, Markel TA, Brown JW, Meldrum DR. Stem cell transplantation as a therapeutic approach to organ failure. J Surg Res. 2005;129:152–160
  7. Crisostomo PR, Wang Y, Markel TA, Wang M, Lahm T, Meldrum DR. Human mesenchymal stem cells stimulated by TNF-{alpha}, LPS, or hypoxia produce growth factors by an NF{kappa}B- but not JNK-dependent mechanism. Am J Physiol Cell Physiol. 2008;294:C675–C682
  8. Patel KM, Crisostomo P, Lahm T, Markel TA, Herring C, Wang M, et al. Mesenchymal stem cells attenuate hypoxic pulmonary vasoconstriction by a paracrine mechanism. J Surg Res. 2007;143:281–285
  9. Meldrum KK, Meldrum DR, Hile KL, Burnett AL, Harken AH. A novel model of ischemia in renal tubular cells which closely parallels in vivo injury. J Surg Res. 2001;99:288–293
  10. Wang M, Tsai BM, Crisostomo PR, Meldrum DR. Pretreatment with adult progenitor cells improves recovery and decreases native myocardial proinflammatory signaling after ischemia. Shock. 2006;25:454–459
  11. Xu J, Qu J, Cao L, Sai Y, Chen C, He L, et al. Mesenchymal stem cell-based angiopoietin-1 gene therapy for acute lung injury induced by lipopolysaccharide in mice. J Pathol. 2008;214:472–481
  12. Guillot P, Cook H, Pusey C, Fisk N, Harten S, Moss J, et al. Transplantation of human fetal mesenchymal stem cells improves glomerulopathy in a collagen type I alpha2-deficient mouse. J Pathol. 2008;214:627–636
  13. Ditschkowski M, Einsele H, Schwerdtfeger R, Bunjes D, Trenschel R, Beelen DW, et al. Improvement of inflammatory bowel disease after allogeneic stem-cell transplantation. Transplantation. 2003;75:1745–1747
  14. Aimoto T, Uchida E, Matsushita A, Tabata Y, Takano T, Miyamoto M, et al. Controlled release of basic fibroblast growth factor promotes healing of the pancreaticojejunal anastomosis: a novel approach toward zero pancreatic fistula. Surgery. 2007;142:734–740
  15. Jeschke MG, Bolder U, Finnerty CC, Przkora R, Muller U, Maihofer R, et al. The effect of hepatocyte growth factor on gut mucosal apoptosis and proliferation, and cellular mediators after severe trauma. Surgery. 2005;138:482–489
  16. Rollwagen FM, Madhavan S, Singh A, Li YY, Wolcott K, Maheshwari R. IL-6 protects enterocytes from hypoxia-induced apoptosis by induction of bcl-2 mRNA and reduction of fas mRNA. Biochem Biophys Res Commun. 2006;347:1094–1098
  17. Wang Q, Sun X, Pritts TA, Wong HR, Hasselgren PO. Induction of the stress response increases interleukin-6 production in the intestinal mucosa of endotoxaemic mice. Clin Sci (Lond). 2000;99:489–496
  18. Conner EA, Wirth PJ, Kiss A, Santoni-Rugiu E, Thorgeirsson SS. Growth inhibition and induction of apoptosis by HGF in transformed rat liver epithelial cells. Biochem Biophys Res Commun. 1997;236:396–401
  19. Wang Y, Ahmad N, Wani MA, Ashraf M. Hepatocyte growth factor prevents ventricular remodeling and dysfunction in mice via Akt pathway and angiogenesis. J Mol Cell Cardiol. 2004;37:1041–1052
  20. Schwartz MZ, Kato Y, Yu D, Lukish JR. Growth-factor enhancement of compromised gut function following massive small-bowel resection. Pediatr Surg Int. 2000;16:174–175
  21. von Dobschuetz E, Meyer S, Thorn D, Marme D, Hopt UT, Thomusch O. Targeting vascular endothelial growth factor pathway offers new possibilities to counteract microvascular disturbances during ischemia/reperfusion of the pancreas. Transplantation. 2006;82:543–549
  22. Wang Y, Haider HK, Ahmad N, Xu M, Ge R, Ashraf M. Combining pharmacological mobilization with intramyocardial delivery of bone marrow cells over-expressing VEGF is more effective for cardiac repair. J Mol Cell Cardiol. 2006;40:736–745
  23. Gerber HP, Malik AK, Solar GP, Sherman D, Liang XH, Meng G, et al. VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Nature. 2002;417:954–958
  24. Fu XB, Li XK, Wang T, Cheng B, Sheng ZY. Enhanced anti-apoptosis and gut epithelium protection function of acidic fibroblast growth factor after cancelling of its mitogenic activity. World J Gastroenterol. 2004;10:3590–3596
  25. Li P, Nijhawan D, Wang X. Mitochondrial activation of apoptosis. Cell. 2004;116:S57–S59

 Presented at the 69th Annual Meeting of the Society of University Surgeons, Fort Myers, Florida, February 3–6, 2009.

 Supported in part by research grants from the National Institutes of Health (R01GM070628 [to D.R.M.], R01HL085595 [to D.R.M.], F32HL093987 [to B.R.W.], F32HL085982 [to T.A.M.], F32HL092718 [to A.M.A.], and F32HL092719 [to J.L.H.]).

 B.R.W. and T.A.M. contributed equally to this publication.

PII: S0039-6060(09)00233-5

doi: 10.1016/j.surg.2009.03.031

Surgery
Volume 146, Issue 2 , Pages 190-197 , August 2009

Article Tools

* Image Usage & Resolution