Surgery
Volume 146, Issue 5 , Pages 888-895 , November 2009

Presence of minute cancer cell dissemination in peritoneal lavage fluid detected by reverse transcription PCR is an independent prognostic factor in patients with resectable pancreatic cancer

  • Hidetoshi Eguchi, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • Corresponding Author InformationReprint requests: Hidetoshi Eguchi, MD, Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan.
    • ,
  • Hiroaki Ohigashi, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Hidenori Takahashi, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Masahiko Yano, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Masaaki Motoori, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Isao Miyashiro, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Kentaro Kishi, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Masayuki Ohue, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Shingo Noura, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Yosuke Seki, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Terumasa Yamada, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Kunihito Goto, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Takashi Yamamoto

      Affiliations

    • Department of Clinical Laboratory, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Atushi Idota

      Affiliations

    • Department of Clinical Laboratory, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Junko Fujii

      Affiliations

    • Department of Clinical Laboratory, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Hiromu Nakajima, MD

      Affiliations

    • Department of Clinical Laboratory, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Tomoyuki Yamasaki, MD

      Affiliations

    • Department of Clinical Laboratory, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    • ,
  • Osamu Ishikawa, MD

      Affiliations

    • Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan

,Accepted 10 April 2009.

References 

  1. Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ. Cancer statistics, 2007. CA Cancer J Clin. 2007;57:43–66
  2. Parkin DM, Bray FI, Devesa SS. Cancer burden in the year 2000. The global picture. Eur J Cancer. 2001;37(Suppl 8):S4–S66
  3. Ishikawa O, Ohhigashi H, Sasaki Y, Kabuto T, Fukuda I, Furukawa H, et al. Practical usefulness of lymphatic and connective tissue clearance for the carcinoma of the pancreas head. Ann Surg. 1988;208:215–220
  4. Yeo CJ, Cameron JL, Lillemoe KD, Sitzmann JV, Hruban RH, Goodman SN, et al. Pancreaticoduodenectomy for cancer of the head of the pancreas. 201 patients. Ann Surg. 1995;221:721–733
  5. Sobin LH, Wittekind C. TNM classification of malignant tumours. 6th ed. Hoboken, NJ: John Wiley & Sons; 2002;250 pp
  6. Ferrone CR, Haas B, Tang L, Coit DG, Fong Y, Brennan MF, et al. The influence of positive peritoneal cytology on survival in patients with pancreatic adenocarcinoma. J Gastrointest Surg. 2006;10:1347–1353
  7. Jimenez RE, Warshaw AL, Fernandez-Del Castillo C. Laparoscopy and peritoneal cytology in the staging of pancreatic cancer. J Hepatobiliary Pancreat Surg. 2000;7:15–20
  8. Leach SD, Rose JA, Lowy AM, Lee JE, Charnsangavej C, Abbruzzese JL, et al. Significance of peritoneal cytology in patients with potentially resectable adenocarcinoma of the pancreatic head. Surgery. 1995;118:472–478
  9. Yamada S, Takeda S, Fujii T, Nomoto S, Kanazumi N, Sugimoto H, et al. Clinical implications of peritoneal cytology in potentially resectable pancreatic cancer: positive peritoneal cytology may not confer an adverse prognosis. Ann Surg. 2007;246:254–258
  10. Meszoely IM, Lee JS, Watson JC, Meyers M, Wang H, Hoffman JP. Peritoneal cytology in patients with potentially resectable adenocarcinoma of the pancreas. Am Surg. 2004;70:208–214
  11. Yachida S, Fukushima N, Sakamoto M, Matsuno Y, Kosuge T, Hirohashi S. Implications of peritoneal washing cytology in patients with potentially resectable pancreatic cancer. Br J Surg. 2002;89:573–578
  12. Nakao A, Oshima K, Takeda S, Kaneko T, Kanazumi N, Inoue S, et al. Peritoneal washings cytology combined with immunocytochemical staining in pancreatic cancer. Hepatogastroenterology. 1999;46:2974–2977
  13. Shield P. Peritoneal washing cytology. Cytopathology. 2004;15:131–141
  14. Guller U, Zajac P, Schnider A, Bosch B, Vorburger S, Zuber M, et al. Disseminated single tumor cells as detected by real-time quantitative polymerase chain reaction represent a prognostic factor in patients undergoing surgery for colorectal cancer. Ann Surg. 2002;236:768–776
  15. Kodera Y, Nakanishi H, Ito S, Yamamura Y, Kanemitsu Y, Shimizu Y, et al. Quantitative detection of disseminated free cancer cells in peritoneal washes with real-time reverse transcriptase-polymerase chain reaction: a sensitive predictor of outcome for patients with gastric carcinoma. Ann Surg. 2002;235:499–506
  16. Katsuragi K, Yashiro M, Sawada T, Osaka H, Ohira M, Hirakawa K. Prognostic impact of PCR-based identification of isolated tumour cells in the peritoneal lavage fluid of gastric cancer patients who underwent a curative R0 resection. Br J Cancer. 2007;97:550–556
  17. Ito S, Nakanishi H, Kodera Y, Mochizuki Y, Tatematsu M, Yamamura Y. Prospective validation of quantitative CEA mRNA detection in peritoneal washes in gastric carcinoma patients. Br J Cancer. 2005;93:986–992
  18. Dalal KM, Woo Y, Galanis C, Gonen M, Tang L, Allen P, et al. Detection of micrometastases in peritoneal washings of pancreatic cancer patients by the reverse transcriptase polymerase chain reaction. J Gastrointest Surg. 2007;11:1598–1606
  19. Hoffmann K, Kerner C, Wilfert W, Mueller M, Thiery J, Hauss J, et al. Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood, bone marrow and peritoneal lavage of pancreatic carcinoma patients. World J Gastroenterol. 2007;13:257–263
  20. Ishikawa O, Wada H, Ohigashi H, Doki Y, Yokoyama S, Noura S, et al. Postoperative cytology for drained fluid from the pancreatic bed after “curative” resection of pancreatic cancers: does it predict both the patient's prognosis and the site of cancer recurrence?. Ann Surg. 2003;238:103–110
  21. Further evidence of effective adjuvant combined radiation and chemotherapy following curative resection of pancreatic cancer. Gastrointestinal Tumor Study Group. Cancer. 1987;59:2006–2010
  22. Yamamoto K, Shimada S, Hirota M, Yagi Y, Matsuda M, Baba H. EIPL (extensive intraoperative peritoneal lavage) therapy significantly reduces peritoneal recurrence after pancreatectomy in patients with pancreatic cancer. Int J Oncol. 2005;27:1321–1328
  23. Sugarbaker PH. Peritoneum as the first-line of defense in carcinomatosis. J Surg Oncol. 2007;95:93–96
  24. Tsujimoto M, Nakabayashi K, Yoshidome K, Kaneko T, Iwase T, Akiyama F, et al. One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. Clin Cancer Res. 2007;13:4807–4816

PII: S0039-6060(09)00296-7

doi: 10.1016/j.surg.2009.04.021

Surgery
Volume 146, Issue 5 , Pages 888-895 , November 2009

Article Tools

* Image Usage & Resolution