The utility of [11C] dihydrotetrabenazine positron emission tomography scanning in assessing β-cell performance after sleeve gastrectomy and duodenal-jejunal bypass
Presented at the 2009 meeting of the Society of University Surgeons.
Accepted 20 August 2009. published online 14 October 2009.
Background
The aim of this study was to evaluate the effect of sleeve gastrectomy (SG) and duodenal-jejunal bypass (DJB) on glucose homeostasis and to evaluate the utility of positron emission tomography (PET) scanning for assessing β-cell mass.
Methods
Goto-Kakizaki rats were divided into 4 groups: control, sham, SG, or DJB. Oral glucose tolerance, insulin, and glucagon-like peptide-1 (GLP-1) were measured before and after surgery. Before and 90 days after treatment, [11C] DTBZ micro PET scanning was performed.
Results
The control and sham animals gained more weight compared with SG and DJB animals (P ≤ .05). Compared with control animals, the glucose area under the curve was lower in DJB animals 30 and 45 days after operations (P ≤ .05). At killing, GLP-1 levels were greater in the DJB group compared with sham and SG (P ≤ .05), whereas insulin levels were greater in both DJB and SG compared with sham (P ≤ .05). With PET scanning, the 90-day posttreatment mean vesicular monoamine transporter type 2 binding index was greatest in the DJB animals (2.45) compared with SG (1.17), both of which were greater than baseline control animals (0.81).
Conclusion
In type 2 diabetic rodents, DJB leads to improved glucose homeostasis and an increase in VMAT2 density as measured by PET scanning.
aDivision of Metabolic, Endocrine and Minimally Invasive Surgery. Department of Surgery, Mount Sinai Medical Center, New York, NY
bDivision of Gastrointestinal and Endocrine Surgery, Department of Surgery, College of Physicians and Surgeons of Columbia University, New York, NY
cDivision of Endocrinology, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY
dDepartment of Pathology, College of Physicians and Surgeons of Columbia University, New York, NY
Reprint requests: William B. Inabnet, MD, Chief, Division of Metabolic, Endocrine and Minimally Invasive Surgery, Mount Sinai Medical Center, 5 East 98th Street, Box 1259, New York, NY 10029.
ABSTRACT