Surgery
Volume 120, Issue 1 , Pages 16-22 , July 1996

Wound closure with human keratinocytes cultured on a polyurethane dressing overlaid on a cultured human dermal replacement

    MD
  • Hans O. Rennekampff

      Affiliations

    • Department of Surgery, San Diego, Calif. USA
    • Department of Medicine, San Diego, Calif. USA
    • Department of Orthopedics, University of California, San Diego Medical Center, San Diego, Calif. USA
    • Corresponding Author InformationReprint requests: Oliver Rennekampff, MD, Department of Surgery 8896, University of California, San Diego Medical Center, 200 W. Arbor Dr., San Diego, CA 92103.
    • H. O. Rennekampff is supported by a grant from Deutsche Forschungsgemeinschaft.
    • , MD
  • John F. Hansbrough

      Affiliations

    • Department of Surgery, San Diego, Calif. USA
    • Department of Medicine, San Diego, Calif. USA
    • Department of Orthopedics, University of California, San Diego Medical Center, San Diego, Calif. USA
    • , BS
  • Verena Kiessig

      Affiliations

    • Department of Surgery, San Diego, Calif. USA
    • Department of Medicine, San Diego, Calif. USA
    • Department of Orthopedics, University of California, San Diego Medical Center, San Diego, Calif. USA
    • ,
  • Salwan Abiezzi

      Affiliations

    • Department of Surgery, San Diego, Calif. USA
    • Department of Medicine, San Diego, Calif. USA
    • Department of Orthopedics, University of California, San Diego Medical Center, San Diego, Calif. USA
    • , MD
  • Virgil Woods Jr.

      Affiliations

    • Department of Surgery, San Diego, Calif. USA
    • Department of Medicine, San Diego, Calif. USA
    • Department of Orthopedics, University of California, San Diego Medical Center, San Diego, Calif. USA

,Accepted 11 October 1995.

References 

  1. Hansbrough JF. Wound coverage with biologic dressings and cultured skin substitutes. Austin: RG Landes Inc; 1992;
  2. Marchisio PC, Bondanza S, Cremona O, Cancedda R, DeLuca M. Polarized expression of integrin receptors (α6β4, α2β1, α3β1, and αVβ5) and their relationship with the cytoskeleton and basement membrane matrix in cultured human keratinocytes. J Cell Biol. 1991;112:761–773
  3. Rue LW, Cioffi WG, McManus WF, Pruitt BA. Wound closure and outcome in extensively burned patients treated with cultured autologous keratinocytes. J Trauma. 1993;34:662–668
  4. Desai MH, Mlakar JM, McCauley RL, et al. Lack of long-term durability of cultured keratinocyte burn-wound coverage: a case report. J Burn Care Rehabil. 1991;12:540–545
  5. Poumay Y, Boucher F, Degen A, Leloup R. Inhibition of basal cell proliferation during storage of detached epidermal keratinocyte sheets. Acta Derm Venereol. 1991;71:195–198
  6. Hynes RO. Integrins: a family of cell surface receptors. Cell. 1987;48:549–554
  7. Marchisio PC, Bondanza S, Cremona O, et al. Polarized expression of integrin receptors and their relationship with the cyto-skeleton and basement membrane matrix in cultured human keratinocytes. J Cell Biol. 1991;112:761–773
  8. Stenn K, Link R, Moellmann G, et al. Dispase, a neutral protease from Bacillus polymyxa, is a powerful fibronectinase and type IV collagenase. J Invest Dermatol. 1989;93:287–290
  9. Poumay Y, Leclercq-Smekens M, Grailly S, et al. Specific internalization of basal membrane domains containing the integrin α6β4 in Dispase-detached cultured human keratinocytes. Eur J Cell Biol. 1993;60:12–20
  10. Hotchin NA, Watt FM. Transcriptional and post-translational regulation of β1 integrin expression during keratinocyte terminal differentiation. J Biol Chem. 1992;267:14852–14858
  11. Adams JC, Watt FM. Changes in keratinocyte adhesion during terminal differentiation: reduction in fibronectin binding precedes α5β1 integrin loss from the cell surface. Cell. 1990;63:425–435
  12. Gailit J, Clark RAF. Wound repair in the context of extracellular matrix. Curr Opin Cell Biol. 1994;6:717–725
  13. Takashima A, Grinnel F. Fibronectin-mediated keratinocyte migration and initiation of fibronectin receptor function in vitro. J Invest Dermatol. 1985;85:304–308
  14. Lamke LO, Liljedahl SO. Evaporative water loss from burns, grafts and donor sites. Scand J Plast Surg. 1971;5:17–22
  15. Briggaman RA, Wheeler CE. Epidermal-dermal interactions in adult human skin: role of dermis in epidermal maintenance. J Invest Dermatol. 1968;51:454–465
  16. Woodley DT, Peterson HD, Herzog SR, et al. Burn wounds resurfaced by cultured epidermal autografts show abnormal reconstitution of anchoring fibrils. JAMA. 1988;259:2566–2571
  17. Hansbrough JF, Morgan JL, Greenleaf GE, Bartel R. Composite grafts of human keratinocytes grown on a polyglactin mesh-cultured fibroblast dermal substitute function as a bilayer skin replacement in full-thickness wounds on athymic mice. J Burn Care Rehabil. 1993;14:485–494
  18. Hansbrough JF, Doré C, Hansbrough WB. Clinical trials of a living dermal tissue replacement placed beneath meshed, split-thickness skin grafts on excised burn wounds. J Burn Care Rehabil. 1992;13:519–529

PII: S0039-6060(96)80235-2

Surgery
Volume 120, Issue 1 , Pages 16-22 , July 1996

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