Research Article| Volume 51, ISSUE 5, P639-644, May 1962

In vitro selection of chemotherapeutic agents for perfusion therapy of human cancer

      This paper is only available as a PDF. To read, Please Download here.


      A method to aid in the selection of agents to be used for perfusion chemotherapy of human cancers is presented. The test establishes the sensitivity of the patient's tumor to chemotherapeutic agents in vitro. Explants of the tumor, growing in tissue culture, are exposed to several drugs and the cytotoxic effect of each is established. One of the drugs tested is then used in the perfusion treatment of that patient's tumor.
      Of 48 isolation-perfusion operations performed, a comparison of the clinical effects with the tissue culture results was possible in 16. There was a positive correlation between the in vivo and in vitro results in 13 of the 16 cases.
      The results of this initial study suggest that this tissue culture sensitivity test may be a useful tool in the selection of drugs for perfusion.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Surgery
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Antikajian G.
        • Wright L.T.
        • Plummer J.I.
        • Weintraub S.
        The effect of triethylene melamine, Aureomycin and some 4-amino derivatives of folic acid on tissues in vitro.
        J. Nat. Cancer Inst. 1951; 12: 269
        • Blakemore W.S.
        • McKenna J.M.
        • Mc-Allister R.M.
        • Coriell L.L.
        The cytotoxic metabolic inhibition test for screening compounds for antitumor activity.
        in: Proc. Am. A. Cancer Res. 3. 1960: 96
        • Cobb J.P.
        Tissue culture observations of the effects of chemotherapeutic agents on human tumors.
        Tr. New York Acad. Sc. 1955; 17: 237
        • Cobb J.P.
        • Walker D.G.
        Effect of actinomycin D on tissue cultures of normal and neoplastic cells.
        J. Nat. Cancer Inst. 1958; 21: 263
        • Cobb J.P.
        • Walker D.G.
        Studies on human melanoma cells in tissue culture. I. Growth characteristics and cytology.
        Cancer Res. 1960; 20: 858
        • Cobb J.P.
        • Walker D.G.
        Comparative chemotherapy studies on primary short-term cultures of human normal, benign, and malignant tumor tissues—a five year study.
        Cancer Res. 1961; 21: 583
        • Creech Jr., O.
        • Krementz E.T.
        • Ryan R.F.
        • Winblad J.N.
        Regional perfusion utilizing an extracorporeal circuit.
        Ann. Surg. 1958; 148: 616
        • Eagle H.
        • Foley G.E.
        Cytotoxic action of carcinolytic agents in tissue culture.
        Am. J. Med. 1956; 21: 739
        • Eagle H.
        • Foley G.E.
        Cytotoxicity in human cell cultures as primary screen for detection of anti-tumor agents.
        Cancer Res. 1958; 18: 1017
        • Golomb F.M.
        The selection of drugs for perfusion.
        Cancer Chemotherap. Rep. 1960; 10: 53
        • Golomb F.M.
        • Wright J.C.
        • Cobb J.P.
        • Gumport S.L.
        • Postel A.
        • Safadi D.
        The chemotherapy of human solid tumors by perfusion technics.
        in: Proc. Am. A. Cancer Res. 3. 1960: 92
        • Hu F.
        Cytological studies of human pigment cells in tissue culture.
        in: Gordon M. Pigment Cell Biology. Academic Press, Inc, New York1959: 147-158
        • McAllister R.
        • Grunmeier P.W.
        • Coriell L.L.
        • Blakemore W.S.
        A quantitative technique for the measurement of in vitro cytotoxicity of 5-fluor-2-deoxyuridine (FUDR), nitrogen mustard (HN2), and other antitumor agents.
        Cancer. 1959; 12: 939
        • McDonald G.O.
        • Cole W.H.
        The use of human tumors in primary culture for testing anticancer compounds.
        in: S. Forum. 10. 1959: 67
        • Schrek R.
        A method for screening chemotherapeutic agents against normal and leukemic cells of man.
        in: Proc. Am. A. Cancer Res. 3. 1960: 148
        • Toplin I.
        A tissue culture cytotoxicity test for large-scale cancer chemotherapy screening.
        Cancer Res. 1959; 19: 959
        • Wright J.C.
        • Cobb J.P.
        • Gumport S.L.
        • Golomb F.M.
        • Safadi D.
        Investigation of the relation between clinical and tissue culture response to chemotherapeutic agents on human cancer.
        New England J. Med. 1957; 257: 1207