Society of University Surgeons| Volume 52, ISSUE 1, P213-219, July 1962

Antibodies reactive with antigens derived from HeLa and J111 in patients with malignancies

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      • 1.
        1. Serologically distinct antigens(G) have been obtained by fluorocarbon extraction of cells from the line tissue culture cells, HeLa and J111, originating from human malignancies.
      • 2.
        2. Antibodies from 36 per cent of 257 patients with malignant tumors were reactive with the antigen(G) from HeLa and antibodies from 14 per cent of 114 patients from the same group were reactive with the antigen(G) from J111, but were absent in the sera of 180 patients without known malignancies.
      • 3.
        3. While no correlation has been made between the time of onset of the disease or the pathologist's classification of the lesions, antibodies were found more frequently in patients with metastases. The antibodies appear in patients with a wide variety of carcinomas in various organs and with lesser frequency in patients with sarcomas. With certain tumors there is discernible a pattern of the frequency of occurrence of antibody.
      • 4.
        4. After inoculation with melanoma antigen(G) 3 of 4 patients with far-advanced malignant melanoma had measurable increases in antibody where little or no antibody was previously detectable. One gave a secondary antibody response following inoculation with an antigen(G) extracted from his own tumor.
      • 5.
        5. After the fluorocarbon treatment described, trichloroacetic acid-precipitable material remained in the supernatant of each of 115 malignant tumors of humans tested and none has been found after treatment of normal tissues or a few benign tumors. Serologically different materials are recovered from human fetal tissues and malignant tumors of cattle and dogs.
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