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Abstract
Plasma arginine amidase activity was measured for 4 consecutive days in normal subjects,
in hospitalized patients not undergoing operation, and in patients undergoing a variety
of surgical procedures. Mean amidase activity was distinctly higher in both patient
groups than in normal subjects, but there was considerably greater daily variation
in enzyme activity of patients in the operative group than in that of normal subjects
and the patient group not undergoing operation. Abnormal amidase activity was found
in 10 percent of patients in the immediate preoperative period and in 33 percent of
patients in the 4 day postoperative period. The type and magnitude of operation did
not influence postoperative amidase activity. Plasma amylase was elevated postoperatively
less frequently and to a lesser extent than amidase, and there was no correlation
in the activities of the 2 enzymes. Data on the substrate specificity and activation
behavior of several enzymes possibly responsible for the amidase activity of plasma
are presented and suggest that this activity is due to either trypsin or an unidentified
enzyme. The significance of the increased activity of this enzyme in the blood of
postoperative patients is not known but is possibly a manifestation or reflection
of the proteolytic phenomena associated with tissue injury and inflammation.
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References
- Proteolysis, fibrinolysis, and coagulation. Significance in thrombolytic therapy.Circulation. 1962; 26: 596
- The specificity of trypsin.J. Biol. Chem. 1939; 127: 643
- A new method for the determination of the amidase activity of trypsin: Kinetics of the hydrolysis of benzoyl-Largineamide.Biochem. J. 1955; 59: 506
- Observations on fibrinolysis: Experimental activity produced by exercise or adrenalin.Lancet. 1947; 1: 402
- Clinical laboratory methods.in: ed. 5. The C. V. Mosby Co.,, St. Louis1957: 349
- The colorimetric determination of arginine ester hydrolysis by human sera.J. Lab. & Clin. Med. 1960; 55: 616
- The molecular kinetics of trypsin action.J. Am. Chem. Soc. 1941; 63: 2971
- Enzymatic investigations of partially purified hog kallikrein.Nature. 1963; 197: 1300
- Antiproteolytic activity of human serum with particular reference to its changes in the presence and considerations of its use for detection of malignant neoplasia.in: ed. 2. Proc. Soc. Exper. Biol. & Med. 69. 1948: 276
- Variations in the proteolytic and antiproteolytic reactions of serum: Effect of disease, trauma, x-ray, anaphylactic shock, ACTH, and cortisone.J. Lab. & Clin. Med. 1952; 39: 105
- A micromethod for determination of bradykininogen under several conditions.Ann. New York Acad. Sc. 1963; 104: 77
- Biochemical determinants of microbial diseases.Harvard University Press, Cambridge, Mass.,1954
- Bradykinin and its mode of release.Ann. New York Acad. Sc. 1963; 104: 35
- Observations on the inactivation of fibrinolytic activity in shed blood.Clin. Sc. 1952; 11: 309
- The coagulant action of crystalline trypsin, cephalin and lung extracts.Am. J. Physiol. 1939; 126: 661
- Enzymes and blood clotting. I. Trypsin as an accessory factor.J. Clin. Invest. 1960; 39: 1942
- On the proteolytic enzymes of animal tissues. I. Beef spleen.J. Biol. Chem. 1939; 130: 19
- Involvement of a complementlike factor in the activation of blood protease.J. Immunol. 1952; 69: 597
- Increased plasma proteolytic activity due to arginine amidase in patients with pancreatitis.New England J. Med. 1963; 268: 851
- Plasma arginine amidase.New England J. Med. 1963; 269: 53
- Intravenous trypsin, anticoagulant, fibrinolytic and thrombolytic effects.J. Clin. Invest. 1952; 31: 1049
- The plasma amylase. Source, regulation and diagnostic significance.Am. J. Med. 1959; 27: 924
- Activation of plasminogen by trypsin and plasmin.Blood. 1952; 7: 882
- Heat denaturation of plasminogen in the fibrin plate method.Acta. physiol. scandinav. 1953; 27: 371
- Fibrinolysis following operation.Lancet. 1937; 1: 10
- Observations on fibrinolysis. Spontaneous activity associated with surgical operations Trauma, etc.Lancet. 1946; 2: 862
- Biochemical mechanisms in inflammation.in: ed. 2. Charles C Thomas, Publisher, Springfield, Ill.,1956: 83
- Formation and properties of the activator of plasminogen and of human and bovine plasmin.Biochem. J. 1955; 61: 424
- Proteolytic activity of whole human serum following surgery.J. Lab. & Clin. Med. 1957; 49: 377
- Serum trypsin determination in pancreatic disease.J. Lab. & Clin. Med. 1958; 52: 66
- Specific polypeptide substrates in enzymatic determination of pancreatic disease.Surgery. 1959; 46: 30
- Serum trypsin: A new diagnostic test for pancreatic disease.New England J. Med. 1958; 258: 797
- Trypsin activity.New England J. Med. 1963; 269: 644
- Some considerations of multiple specificity of proteolytic enzymes.Ann. New York Acad. Sc. 1957; 68: 11
- Experiences with inhibitors to the plasminplasminogen system in the human subject.Am. J. Cardiol. 1960; 6: 432
- The specific esterase activity of trypsin.J. Biol. Chem. 1948; 172: 221
- Abnormal plasma proteolytic activity.Surg. Gynec. & Obst. 1954; 99: 679
- (Moderator)Hemostatic mechanisms and proteolysis in shock, Round Table Conference. ed. 2. Fed. Proc. 20. 1961: 209
- The action of thrombin on synthetic substrates.J. Biol. Chem. 1954; 208: 95
- Studies on lysis of experimental pulmonary emboli using trypsin.Surgery. 1953; 34: 501
- Effect of trypsin on blood coagulation and mechanism of its action.J. Lab. & Clin. Med. 1956; 48: 519
- The nature of the mechanism of the shock produced by the injection of trypsin and thrombin.J. Clin. Invest. 1945; 24: 1
- The occurrence of fibrinolysis in shock, with observations on the prothrombin time and the plasma fibrinogen during hemorrhagic shock.Am. J. M. Sc. 1946; 211: 88
- Results and hazards of intravenous administration of crystalline trypsin: Postulated role in blood coagulation.J. A. M. A. 1954; 155: 347
- The action of plasmin on synthetic substrates.J. Biol. Chem. 1954; 208: 85
- The nature of the kallidins released from human plasma by kallikreins and other enzymes.Ann. New York Acad. Sc. 1963; 104: 91
- Action of the kallikreins on synthetic ester substrates.in: ed. 2. Proc. Soc. Exper. Biol. & Med. 107. 1961: 186
- Ueber esteratische Wirkungen von Kallikrein und Trypsin und ihre Hemming durch Kallikreinund Trypsin Inhibitors.Ztschr. f. physiol. chem. 1960; 319: 52
Article info
Publication history
Received:
January 2,
1964
Identification
Copyright
© 1965 Published by Elsevier Inc.
