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Original communication| Volume 69, ISSUE 2, P232-237, February 1971

Gastrin resistance following immunization to the C-terminal tetrapeptide amide of gastrin

  • Bernard M. Jaffe
    Affiliations
    From the Departments of Surgery and Medicine, Washington University School of Medicine St. Louis, Mo., USA

    From the John Cochran Veterans Administration Hospital, St. Louis, Mo., USA

    From the Department of Medicine, University of Florida College of Medicine, Gainesville, Fla., USA
    Search for articles by this author
  • James E. McGuigan
    Footnotes
    Affiliations
    From the Departments of Surgery and Medicine, Washington University School of Medicine St. Louis, Mo., USA

    From the John Cochran Veterans Administration Hospital, St. Louis, Mo., USA

    From the Department of Medicine, University of Florida College of Medicine, Gainesville, Fla., USA
    Search for articles by this author
  • William T. Newton
    Affiliations
    From the Departments of Surgery and Medicine, Washington University School of Medicine St. Louis, Mo., USA

    From the John Cochran Veterans Administration Hospital, St. Louis, Mo., USA

    From the Department of Medicine, University of Florida College of Medicine, Gainesville, Fla., USA
    Search for articles by this author
  • Author Footnotes
    ∗ Recipient of Research Career Development Award 1-k3-H1-19,499 from the National Institutes of Health.
DOI:https://doi.org/10.5555/uri:pii:0039606071901541
Gastrin resistance following immunization to the C-terminal tetrapeptide amide of gastrin
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      Abstract

      Young female Sprague-Dawley rats were immunized against gastrin tetrapeptide conjugated to BGG with a water soluble carbodiimide. The control group consisted of rats similarly immunized with a conjugate identically prepared with carbodiimide and BGG but without tetrapeptide. These animals were used in the perfused stomach bioassay to determine the acid secretory response to either 0.4 or 1.0 μg of gastrin per kilogram. Immunization against tetrapeptide induced virtually total resistance to the acid secretory effects of gastrin; the peak responses were only 16 and 33 percent above the basal rate of acid secretion compared to values for the control-immunized animals of 94 and 190 percent. This resistance was accompanied by the production of antibodies which significantly bound [125I] gastrin in a double antibody radioimmunossay system.
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      Article info

      Publication history

      Accepted: May 14, 1970

      Footnotes

      ☆This work was supported by Grant GM00371 and Research Grant 1 R01 AM 10837 from the National Institutes of Health, by Grant T-394 from the American Cancer Society and by Grant 66-679 from the American Heart Association.

      ☆☆A preliminary report of the work was presented to the Central Society for Clinical Research, Chicago, Ill., in October, 1969.

      Identification

      DOI: https://doi.org/10.5555/uri:pii:0039606071901541

      Copyright

      © 1971 Published by Elsevier Inc.

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