Original communication| Volume 72, ISSUE 5, P722-729, November 1972

Studies in prolonged liver preservation

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      A new liver perfusion preservation system has been described that provides wide flexibility in altering the many factors that may contribute to maintained viability of an excised organ. Mechanical regulation of flow, pulse profile, and pressure through two separate collapsible silicone rubber pumps (hepatic artery and portal vein) result in ability to utilize an erythrocyte-containing perfusate. Addition of adenosine triphosphate (ATP) successfully reversed some of the evidence of cellular damage (potassium leak and oxygen and glucose utilization) but did not provide sufficient viability to permit successful orthotopic transplant of the liver perfused for 24 hours. Monitoring Po2 within the liver substance was more sensitive than measurement of surface pH in the detection of hypoperfusion.
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