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A new liver perfusion preservation system has been described that provides wide flexibility in altering the many factors that may contribute to maintained viability of an excised organ. Mechanical regulation of flow, pulse profile, and pressure through two separate collapsible silicone rubber pumps (hepatic artery and portal vein) result in ability to utilize an erythrocyte-containing perfusate. Addition of adenosine triphosphate (ATP) successfully reversed some of the evidence of cellular damage (potassium leak and oxygen and glucose utilization) but did not provide sufficient viability to permit successful orthotopic transplant of the liver perfused for 24 hours. Monitoring Po2 within the liver substance was more sensitive than measurement of surface pH in the detection of hypoperfusion.
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Accepted: April 17, 1972
☆Supported by grants from the John A. Hartford Foundation, Inc., and the United States Public Health Service (Grant No. 2 RO1 AM12126).
© 1972 Published by Elsevier Inc.