Original communication| Volume 72, ISSUE 5, P737-743, November 1972

Immune responses induced by human malignant melanoma in the rabbit

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      Rabbits were immunized with melanoma from a single individual on three consecutive occasions. The antisera were absorbed with a variety of normal tissues. Following this absorption, the final antisera reacted strongly in immunofluorescence against most melanomas tested in tissue section, with only minimal background fluorescence of normal tissue components. All antisera reacted with cells of four pigment-producing cultured melanoma cell lines. Absorption with whole melanoma completely removed the activity against melanoma cells. The reaction was not related to melanin and was negative against benign mole and other tumor cells tested. The antiserum was not cytotoxic for four cultured melanoma cell lines. It was concluded that the immunized rabbit produced antibodies to an antigen common to most human melanomas.
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