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Original communication| Volume 72, ISSUE 5, P737-743, November 1972

Immune responses induced by human malignant melanoma in the rabbit

DOI:https://doi.org/10.5555/uri:pii:0039606072902565
Immune responses induced by human malignant melanoma in the rabbit
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      Abstract

      Rabbits were immunized with melanoma from a single individual on three consecutive occasions. The antisera were absorbed with a variety of normal tissues. Following this absorption, the final antisera reacted strongly in immunofluorescence against most melanomas tested in tissue section, with only minimal background fluorescence of normal tissue components. All antisera reacted with cells of four pigment-producing cultured melanoma cell lines. Absorption with whole melanoma completely removed the activity against melanoma cells. The reaction was not related to melanin and was negative against benign mole and other tumor cells tested. The antiserum was not cytotoxic for four cultured melanoma cell lines. It was concluded that the immunized rabbit produced antibodies to an antigen common to most human melanomas.
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      References

        • Alexander P.
        Immunotherapy of cancer: Experiments with primary tumors and syngeneic grafts.
        Progr. Exp. Tumor Res. 1968; 10: 22
        • Barrett M.K.
        A critical analysis of tumor immunity.
        J. Chronic Dis. 1958; 8: 136
        • Bartlett P.C.
        Changes in the immunogenetic relationship of the B16 melanoma in the C57 BL/6 mouse.
        Bull. Tulane Med. Fac. 1967; 26: 199
        • Everson T.C.
        • Cole W.H.
        Spontaneous regression of cancer.
        W. B. Saunders Company, Philadelphia1966
        • Gold P.
        • Freedman S.O.
        Demonstration of tumor-specific antigens in human colonic carcinomata by immunological tolerance and absorption techniques.
        J. Exp. Med. 1965; 121: 439
        • Gold P.
        • Freedman S.O.
        Specific carcinoembryonic antigens of the human digestive system.
        J. Exp. Med. 1966; 122: 467
        • Gray B.K.
        Demonstration of antibody in melanoma patients cytotoxic to human melanoma cells.
        in: ed. 2. Proc. Am. Assoc. Cancer Res. 12. 1971: 79
        • Hauschka T.S.
        Immunologic aspects of cancer—a review.
        Cancer Res. 1952; 12: 615
        • Howard G.H.
        • Spalter H.F.
        Study of autoimmune serologic reactions to ocular melanoma.
        Arch. Ophthal. (Chicago). 1966; 76: 399
        • Humphrey L.J.
        • Jewell W.R.
        • Murray D.R.
        • Griffen W.O.
        Immunotherapy for the patient with cancer.
        Ann. Surg. 1971; 173: 47
        • Ikonopisov R.L.
        • Lewis M.G.
        • Hunter-Craig I.D.
        • Bodenham D.C.
        • Phillips T.M.
        • Cooling C.I.
        • Proctor J.
        • Hamilton F.G.
        • Alexander P.
        Autoimmunization with irradiated tumor cells in human malignant melanoma.
        Br. Med. J. 1970; 2: 752
        • Klein G.
        Tumor antigens.
        Ann. Rev. Microbiol. 1966; 20: 223
        • Krementz E.T.
        • Samuels M.S.
        • Wallace J.H.
        • Benes E.
        Clinical experiences in immunotherapy of cancer.
        Surg. Gynecol. Obstet. 1971; 133: 209
        • Lewis M.G.
        Possible immunological factors in human malignant melanoma in Uganda.
        Lancet. 1967; 2: 1921
        • Lewis M.G.
        • Ikonopisov R.L.
        • Nairn R.C.
        • Phillips T.M.
        • Hamilton F.G.
        • Bodenham D.C.
        • Alexander P.
        Tumor-specific antibodies in human malignant melanoma and their relationship to the extent of disease.
        Br. Med. J. 1969; 3: 547
        • Miller G.L.
        • Wilson J.E.
        Specificity of cytotoxic heterologous antiserum to cultured human cancer cells.
        J. Immunol. 1968; 101: 1078
        • Morton D.L.
        • Malmgrem R.A.
        • Holmes E.C.
        • Ketcham A.S.
        Demonstration of antibodies against human malignant melanoma by immunofluorescence.
        Surgery. 1968; 64: 233
        • Muna N.M.
        • Marcus S.
        • Smart C.
        Detection of immunofluorescence of antibodies specific for human malignant melanoma cells.
        Cancer. 1969; 23: 88
        • Nadler S.H.
        • Moore G.E.
        Clinical immunologic study of malignant disease. Response to Tumor Transplant and Transfer of Leukocytes.
        Ann. Surg. 1966; 164: 482
        • Nairn R.C.
        Fluorescent protein tracing.
        ed. 2. E. & S. Livingstone, Ltd, Edinburgh1964
        • Old L.J.
        • Boyse E.A.
        Immunology of experimental tumors.
        Ann. Rev. Med. 1964; 15: 167
        • Pellegrino A.
        • Reisfeld R.A.
        Biochemical characterization of HL-A antigens.
        in: Fed. Proc. 30. 1971: 691
        • Reisfeld R.A.
        • Kahan B.D.
        Biological and chemical characterization of human histocompatibility antigens.
        in: Fed. Proc. 29. 1970: 2034
        • Shulman N.R.
        • Marder V.J.
        • Hiller M.C.
        • Collier E.M.
        Platelet leukocyte antigens and their antibodies: Serological, physiological and clinical studies.
        Progr. Hematol. 1961; 4: 222
        • Southam C.M.
        Relationship of immunology to cancer: A review.
        Cancer Res. 1960; 20: 271
        • Southam C.M.
        Evidence for cancer-specific antigens in man.
        Progr. Exp. Tumor Res. 1967; 9: 1

      Article info

      Publication history

      Accepted: April 17, 1972

      Footnotes

      ☆Supported by U. S. Public Health Research Grant No. 5T01 CA05108 and No. 5P02 CA05837 and a Research Grant from the National Science Foundation.

      Identification

      DOI: https://doi.org/10.5555/uri:pii:0039606072902565

      Copyright

      © 1972 Published by Elsevier Inc.

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