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Rabbits were immunized with melanoma from a single individual on three consecutive occasions. The antisera were absorbed with a variety of normal tissues. Following this absorption, the final antisera reacted strongly in immunofluorescence against most melanomas tested in tissue section, with only minimal background fluorescence of normal tissue components. All antisera reacted with cells of four pigment-producing cultured melanoma cell lines. Absorption with whole melanoma completely removed the activity against melanoma cells. The reaction was not related to melanin and was negative against benign mole and other tumor cells tested. The antiserum was not cytotoxic for four cultured melanoma cell lines. It was concluded that the immunized rabbit produced antibodies to an antigen common to most human melanomas.
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Accepted: April 17, 1972
☆Supported by U. S. Public Health Research Grant No. 5T01 CA05108 and No. 5P02 CA05837 and a Research Grant from the National Science Foundation.
© 1972 Published by Elsevier Inc.