Society of University Surgeons| Volume 74, ISSUE 2, P163-170, August 1973

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Prospective in vitro prediction of enhancement of canine renal allografts

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      The mixed lymphocyte culture when carried out in two stages (discriminative mixed lymphocyte culture—DMLC) can differentiate between cytotoxic vs. blocking antibody. We have employed the DMLC to predict by an in vitro assay at what point a state of in vivo enhancement had occurred. Dogs undergoing unmodified rejection of heterotopic renal allografts rejected their kidneys in five to nine days. Following rejection cytotoxic antibody could be detected both by microlymphocytotoxicity testing and by nonreversible blocking of the DMLC assay. The DMLC predicted the presence of cytotoxic antibody weeks after the microlymphocytotoxicity assay was no longer positive. Transplants from the donor of the first kidney, if carried out during this time interval, were rejected in less than six days. Approximately two months following transplantation the DMLC assay became reversible. Kidney transplants from the original donor performed during this time interval were not rejected before ten days. Column chromatography isolated an active immunoglobulin component responsible for the in vitro blocking in the IgG fraction. A means of prospectively predicting in vivo enhancement may be at hand.
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        • Amos D.B.
        • Bashir H.
        • Boyle W.
        • Mac-Queen M.
        • Tiilikainen A.
        A simple micro cytotoxicity test.
        Transplantation. 1969; 7: 220
        • Batchelor J.R.
        • Silverman M.S.
        Transplant., Ciba Foundation Symposium. 1961: 216
        • Bloom E.T.
        • Hildemann W.H.
        Mechanisms of tumor-specific enhancement versus resistance toward a methyl holanthrene-induced murine sarcoma.
        Transplantation. 1970; 10: 321
        • Chantler S.M.
        Characteristics of the cellular response in pretreated recipients exhibiting enhanced tumor growth.
        Transplantation. 1967; 5: 1400
        • Cruse J.M.
        • Germany W.W.
        • Dulaney A.D.
        Effects of immune serum and spleen cells on C3Hf/He tumor graft survival in Tennessee Swiss mice.
        J. Lab. Invest. 1965; 14: 1554
        • French M.E.
        • Batchelor J.R.
        Immunological enhancement of rat kidney grafts.
        Lancet. 1969; 2: 1103
        • Gorer P.A.
        Some reactions of H-2 antibodies in vitro and in vivo.
        Ann. N. Y. Acad. Sci. 1958; 73: 707
        • Hattler B.G.
        • Karesch C.
        • Miller J.
        Inhibition of the mixed lymphocyte culture response by antibody following successful human renal transplantation.
        Tissue Antigens. 1971; 1: 270
        • Hattler Jr., B.G.
        • Miller J.
        • Johnson M.C.
        Cellular and humoral factors governing canine mixed lymphocyte cultures after renal transplantation. II. Cellular.
        Transplantation. 1972; 14: 47
        • Hattler B.G.
        • Miller J.
        Blocking antibody in the sera and on the kidneys of successful human transplants.
        Transplant. Proc. 1973; 5: 655
        • Irvin III, G.L.
        • Eustace J.C.
        A study of tumor allograft-sensitized lymph nodes in mice.
        J. Immunol. 1971; 106: 956
        • Lucas Z.J.
        • Markley J.
        • Travis M.
        Immunologic enhancement of renal allograft in the rat. I. Dissociation of graft survival and antibody response.
        in: Fed. Proc. 29. 1970: 2041
        • Marquet R.H.
        • Heystek G.A.
        • Tinbergen W.J.
        Specific inhibition of organ allograft rejection by donor blood.
        Transplant. Proc. 1971; 3: 708
        • Miller J.
        • Hattler B.
        • Davis M.
        • Johnson M.C.
        Cellular and humoral factors governing canine mixed lymphocyte cultures after renal transplantation. I. Antibody.
        Transplantation. 1971; 12: 65
        • Miller J.
        • Hattler Jr., B.G.
        • Johnson M.C.
        Cellular and humoral factors governing canine mixed lymphocyte cultures after renal transplantation. III. Further studies with antibody.
        Transplantation. 1972; 14: 57
        • Mullen Y.
        • Hildemann W.H.
        Kidney transplantation genetics and enhancement in rats.
        Transplant. Proc. 1971; 3: 669
        • Pierce J.C.
        • Varco R.L.
        Technical considerations in the transplantation of the kidney to the dog's neck.
        J. Surg. Res. 1964; 4: 275
        • Quadracci L.J.
        • Hellstrom I.E.
        • Striker G.E.
        • Marchioro T.M.
        • Hellstrom K.E.
        Immune mechanisms in human recipients of renal allografts.
        Cell. Immunol. 1971; 1: 561
        • Stephens F.O.
        • Cockrum K.C.
        • Scott D.F.
        • Price D.C.
        • Belzer F.O.
        Attempted renal allograft enhancement by pooled kidney antigen pretreatment.
        Ann. Surg. 1972; 175: 41
        • Stuart F.P.
        • Saitoh R.
        • Fitch F.W.
        Rejection of renal allografts: Specific immunologic suppression.
        Science. 1968; 160: 1463
        • White E.
        • Hildemann W.H.
        Kidney versus skin allograft reactions in normal adult rats of inbred strains.
        Transplant. Proc. 1969; 1: 395
        • White E.
        • Hildemann W.H.
        • Mullen Y.
        Chronic kidney allograft reactions in rats.
        Transplantation. 1969; 8: 602
        • Wilson R.E.
        • Maggs P.R.
        • Wanwyck R.
        • Sharponick T.
        • Hall-Allen R.T.J.
        • Luke P.
        • Simonsen S.J.
        Active enhancement of renal allografts in dog and rat by subcellular antigen.
        Transplant. Proc. 1971; 3: 705
        • Wilson R.E.
        • Rippin A.
        • Dagher R.K.
        • Kinneart P.
        • Busch G.J.
        Prolonged canine renal allograft survival after pretreatment with solubilized antigen.
        Transplantation. 1969; 7: 360
        • Yunis E.J.
        • Amos D.B.
        Three closely linked genetic systems relevant to transplantation.
        in: Proc. Nat. Acad. Sci. 68. 1971: 3031