Advertisement
Surgery
Advanced searchSave search
Society of University Surgeons| Volume 74, ISSUE 2, P163-170, August 1973

Download started.

Ok

Prospective in vitro prediction of enhancement of canine renal allografts

DOI:https://doi.org/10.5555/uri:pii:0039606073900494
Prospective in vitro prediction of enhancement of canine renal allografts
Next ArticleClinical segmental pancreatic transplantation with ureter-pancreatic duct anastomosis for exocrine drainage
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      The mixed lymphocyte culture when carried out in two stages (discriminative mixed lymphocyte culture—DMLC) can differentiate between cytotoxic vs. blocking antibody. We have employed the DMLC to predict by an in vitro assay at what point a state of in vivo enhancement had occurred. Dogs undergoing unmodified rejection of heterotopic renal allografts rejected their kidneys in five to nine days. Following rejection cytotoxic antibody could be detected both by microlymphocytotoxicity testing and by nonreversible blocking of the DMLC assay. The DMLC predicted the presence of cytotoxic antibody weeks after the microlymphocytotoxicity assay was no longer positive. Transplants from the donor of the first kidney, if carried out during this time interval, were rejected in less than six days. Approximately two months following transplantation the DMLC assay became reversible. Kidney transplants from the original donor performed during this time interval were not rejected before ten days. Column chromatography isolated an active immunoglobulin component responsible for the in vitro blocking in the IgG fraction. A means of prospectively predicting in vivo enhancement may be at hand.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Surgery
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Amos D.B.
        • Bashir H.
        • Boyle W.
        • Mac-Queen M.
        • Tiilikainen A.
        A simple micro cytotoxicity test.
        Transplantation. 1969; 7: 220
        • Batchelor J.R.
        • Silverman M.S.
        Transplant., Ciba Foundation Symposium. 1961: 216
        • Bloom E.T.
        • Hildemann W.H.
        Mechanisms of tumor-specific enhancement versus resistance toward a methyl holanthrene-induced murine sarcoma.
        Transplantation. 1970; 10: 321
        • Chantler S.M.
        Characteristics of the cellular response in pretreated recipients exhibiting enhanced tumor growth.
        Transplantation. 1967; 5: 1400
        • Cruse J.M.
        • Germany W.W.
        • Dulaney A.D.
        Effects of immune serum and spleen cells on C3Hf/He tumor graft survival in Tennessee Swiss mice.
        J. Lab. Invest. 1965; 14: 1554
        • French M.E.
        • Batchelor J.R.
        Immunological enhancement of rat kidney grafts.
        Lancet. 1969; 2: 1103
        • Gorer P.A.
        Some reactions of H-2 antibodies in vitro and in vivo.
        Ann. N. Y. Acad. Sci. 1958; 73: 707
        • Hattler B.G.
        • Karesch C.
        • Miller J.
        Inhibition of the mixed lymphocyte culture response by antibody following successful human renal transplantation.
        Tissue Antigens. 1971; 1: 270
        • Hattler Jr., B.G.
        • Miller J.
        • Johnson M.C.
        Cellular and humoral factors governing canine mixed lymphocyte cultures after renal transplantation. II. Cellular.
        Transplantation. 1972; 14: 47
        • Hattler B.G.
        • Miller J.
        Blocking antibody in the sera and on the kidneys of successful human transplants.
        Transplant. Proc. 1973; 5: 655
        • Irvin III, G.L.
        • Eustace J.C.
        A study of tumor allograft-sensitized lymph nodes in mice.
        J. Immunol. 1971; 106: 956
        • Lucas Z.J.
        • Markley J.
        • Travis M.
        Immunologic enhancement of renal allograft in the rat. I. Dissociation of graft survival and antibody response.
        in: Fed. Proc. 29. 1970: 2041
        • Marquet R.H.
        • Heystek G.A.
        • Tinbergen W.J.
        Specific inhibition of organ allograft rejection by donor blood.
        Transplant. Proc. 1971; 3: 708
        • Miller J.
        • Hattler B.
        • Davis M.
        • Johnson M.C.
        Cellular and humoral factors governing canine mixed lymphocyte cultures after renal transplantation. I. Antibody.
        Transplantation. 1971; 12: 65
        • Miller J.
        • Hattler Jr., B.G.
        • Johnson M.C.
        Cellular and humoral factors governing canine mixed lymphocyte cultures after renal transplantation. III. Further studies with antibody.
        Transplantation. 1972; 14: 57
        • Mullen Y.
        • Hildemann W.H.
        Kidney transplantation genetics and enhancement in rats.
        Transplant. Proc. 1971; 3: 669
        • Pierce J.C.
        • Varco R.L.
        Technical considerations in the transplantation of the kidney to the dog's neck.
        J. Surg. Res. 1964; 4: 275
        • Quadracci L.J.
        • Hellstrom I.E.
        • Striker G.E.
        • Marchioro T.M.
        • Hellstrom K.E.
        Immune mechanisms in human recipients of renal allografts.
        Cell. Immunol. 1971; 1: 561
        • Stephens F.O.
        • Cockrum K.C.
        • Scott D.F.
        • Price D.C.
        • Belzer F.O.
        Attempted renal allograft enhancement by pooled kidney antigen pretreatment.
        Ann. Surg. 1972; 175: 41
        • Stuart F.P.
        • Saitoh R.
        • Fitch F.W.
        Rejection of renal allografts: Specific immunologic suppression.
        Science. 1968; 160: 1463
        • White E.
        • Hildemann W.H.
        Kidney versus skin allograft reactions in normal adult rats of inbred strains.
        Transplant. Proc. 1969; 1: 395
        • White E.
        • Hildemann W.H.
        • Mullen Y.
        Chronic kidney allograft reactions in rats.
        Transplantation. 1969; 8: 602
        • Wilson R.E.
        • Maggs P.R.
        • Wanwyck R.
        • Sharponick T.
        • Hall-Allen R.T.J.
        • Luke P.
        • Simonsen S.J.
        Active enhancement of renal allografts in dog and rat by subcellular antigen.
        Transplant. Proc. 1971; 3: 705
        • Wilson R.E.
        • Rippin A.
        • Dagher R.K.
        • Kinneart P.
        • Busch G.J.
        Prolonged canine renal allograft survival after pretreatment with solubilized antigen.
        Transplantation. 1969; 7: 360
        • Yunis E.J.
        • Amos D.B.
        Three closely linked genetic systems relevant to transplantation.
        in: Proc. Nat. Acad. Sci. 68. 1971: 3031

      Article info

      Footnotes

      ☆Supported by National Institutes of Health Grant No. CA 14459-01.

      ☆☆Presented at the Thirty-fourth Annual Meeting of the Society of University Surgeons, New Orleans, La., Feb. 8–10, 1973.

      Identification

      DOI: https://doi.org/10.5555/uri:pii:0039606073900494

      Copyright

      © 1973 Published by Elsevier Inc.

      ScienceDirect

      Access this article on ScienceDirect

      The content on this site is intended for healthcare professionals.


      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the Cookie Preference Center for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties.


      RELX