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Abstract
Based upon long-term functional success of kidneys frozen solid with liquid nitrogen,
capable of sustaining the dog's life after contralateral nephrectomy, the following
factors appear important: (1) surgical technique, (2) perfusion, (3) cryoprotectant,
(4) freeze, and (5) thaw parameters. Application of simple freeze-thaw parameters
important to successful cell suspension or thin tissue slice cryopreservation has
failed when applied to the kidney. This study focuses attention upon each factor,
detailing its contribution to successful freeze-thaw of the canine kidney to −22 °
C. Ultimate success of a renal organ bank rests upon retrieval of a viable organ from
-196 ° C. storage.
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References
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Article info
Footnotes
☆Supported by United States Public Health Service Grant No. PH 43-68-99, Freezing and Thawing Whole Organs, and by the Commonwealth Electric Fund.
☆☆Presented at the Thirty-fourth Annual Meeting of the Society of University Surgeons, New Orleans, La., 1973.
Identification
Copyright
© 1973 Published by Elsevier Inc.
