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Abstract
Stroma-free hemoglobin solution has been proposed as a plasma substitute during acute
resuscitation. Such a solution would have the theoretical advantages of carrying and
unloading oxygen as well as expanding the circulating volume. One potential disadvantage
of this solution is its interference with the normal clotting mechanism. The object
of this report is to describe the anticoagulant properties of this solution and its
correction. A mixture of citrated normal plasma and stroma-free hemoglobin was found
to have a prolonged prothrombin time (PT) and a prolonged activated partial thromboplastin
(APTT) time. Factor identification demonstrated a deficiency in Factor VIII activity.
Factor assay revealed 18.5 percent of normal Factor VIII activity following citrated
normal plasma-stroma-free hemoglobin incubation. Elimination of this anticoagulant
activity was accomplished by adsorption of the stroma-free hemoglobin solution with
hydrated aluminum hydroxide gel at 37 ° C. for one hour. We conclude that (1) an anticoagulant
can be identified in stroma-free hemoglobin solution and (2) this anticoagulant activity
can be abolished by adsorption with hydrated aluminum hydroxide gel.
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Article info
Footnotes
☆This study was supported by funds from United States Army Grant No. DA DA-17-71-C-1106.
☆☆Presented at the Thirty-fourth Annual Meeting of the Society of University Surgeons, New Orleans, La., Feb. 8–10, 1973.
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Copyright
© 1973 Published by Elsevier Inc.
