Original communication| Volume 75, ISSUE 2, P266-273, February 1974

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Relative secretion of cholesterol-4-14C in the bile and upper and lower small intestinal washings of the bile fistula rabbit

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      The secretion of circulating labeled cholesterol by a total biliary fistula, by the isolated upper half of the small intestine, and by the isolated lower half of the small intestine was measured in 21 anesthetized rabbits over an average time of 13 hours. From the secreted radioactivity, ratios were calculated and the three key average radioactivity excretion ratios were: bile counts per minute (cpm)/combined total number of counts per minute (bile counts per minute + upper small intestinal counts per minute + lower small intestinal counts per minute), 0.5470; bile counts per minute/total number of small intestinal counts per minute, 1.4948; and jejunal-ileal counts per minute/distal ileal counts per minute, 1.6031. The measured average jejunal-ileal/ distal ileal length ratio was 1.338. It is concluded that in the rabbit under the acute conditions of this experiment: (1) the cholesterol that is secreted in the bile is Math Eq times the amount secreted by the small intestinal mucosa. (2) Small bowel mucosal cholesterol secretion, on the basis of comparison of the jejunal-ileal/distal ileal counts per minute and length ratios, does not appear to be localized to either the upper or the lower half of the small intestine. (3) Bowel mucosal cholesterol secretion is independent of the presence of bile salts in the intestinal lumen.
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        • Abell L.L.
        • Levy B.B.
        • Brodie B.B.
        • Kendall F.E.
        A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity.
        J. Biol. Chem. 1952; 195: 357
        • Beumer H.
        • Hepner F.
        Über die Ausscheidungswege des Cholesterins, mit freundlicher Unterstutzung der Notgemeinschaft.
        Z. Ges. Exp. Med. 1929; 64: 787
        • Borgstrom B.
        • Lindhe B.A.
        • Wlodawer P.
        Absorption and distribution of cholesterol-4-C14 in the rat.
        in: ed. 5. Proc. Soc. Exp. Biol. Med. 99. 1958: 365
        • Buchwald H.
        • Gebhard R.
        Effect of intestinal bypass on cholesterol absorption and blood levels in the rabbit.
        Am. J. Physiol. 1964; 207: 567
        • Buchwald H.
        • Gebhard R.L.
        The essential role of bile in the intestinal absorption of cholesterol.
        Surgery. 1967; 61: 791
        • Buchwald H.
        • Gebhard R.L.
        Localization of bile salt absorption in-vivo in the rabbit.
        Ann. Surg. 1968; 167: 191
        • Burger M.
        • Oeter H.D.
        Über den Cholesteringehalt der menschlichen Darmwand.
        Z. Physiol. Chem. 1929; 182: 141
        • Byers S.O.
        • Friedman M.
        • Gunning B.
        Observations concerning the production and excretion of cholesterol in mammals. XI. The intestinal site of absorption and excretion of cholesterol.
        Am. J. Physiol. 1953; 175: 375
        • Chevreul M.E.
        Note sur la présence de la cholesterine dans la bile de l'homme.
        Mem. Museum Nat. Hist. Nat. Paris. 1824; 11: 239
        • DeFourcroy
        De la substance feuillettée et cristalline contenue dans les calculs biliaries, et de la nature des concretions cystiques cristalisées.
        Ann. Chim. 1789; 3: 242
        • Dietschy J.M.
        • Siperstein M.D.
        Cholesterol synthesis by the gastrointestinal tract: Localization and mechanism of control.
        J. Clin. Invest. 1965; 44: 1311
        • Gebhard R.L.
        • Buchwald H.
        Cholesterol absorption after reversal of the upper and lower halves of the small intestine.
        in: ed. 5. Surg. Forum. 19. 1968: 304
        • Mueller J.H.
        The mechanism of cholesterol absorption.
        J. Biol. Chem. 1916; 27: 463
        • Schoenheimer R.
        • Sperry W.M.
        Sterol secretion and coprosterol formation.
        J. Biol. Chem. 1934; 107: 1
        • Siperstein M.D.
        • Chaikoff I.L.
        C14-cholesterol III excretion of carbons 4 and 26 in feces, urine, and bile.
        J. Biol. Chem. 1952; 198: 93
        • Siperstein M.D.
        • Chaikoff I.L.
        • Reinhardt W.O.
        C14-cholesterol. V. Obligatory function of bile in intestinal absorption of cholesterol.
        J. Biol. Chem. 1952; 198: 111
        • Sperry W.M.
        A study of the relationship of the bile to the fecal lipids with special reference to certain problems of steroi metabolism.
        J. Biol. Chem. 1926–1927; 71: 351
        • Swell L.
        • Trout Jr., E.C.
        • Field Jr., H.
        • Treadwell C.R.
        The role of lymph cholesterol in the regulation of endogenous cholesterol and cholesterol ester synthesis.
        J. Biol. Chem. 1958; 230: 631
        • Swell L.
        • Trout Jr., E.C.
        • Hopper R.
        • Field Jr., H.
        • Treadwell C.R.
        The mechanism of cholesterol absorption.
        Ann. N. Y. Acad. Sci. 1959; 72: 813