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Abstract
Autotransplantation of the islets of Langerhans in pancreatic tissue fragments, prepared
with no attempt to separate exocrine tissue, produced viable, well vascularized implants
in the peritoneal cavity of pigs. We autografted 31 pancreatectomized pigs with pancreatic
preparations in which exocrine tissue either was separated completely, partially,
or not at all. Nine pancreatectomized pigs were used as control animals. On the basis
of the results of the standard serum glucose and insulin level tests and the mean
survival results, the various methods of tissue preparation had no discernible effect.
One month after grafting, the implants from the unseparated pancreatic preparations
contained significant amounts of insulin and cells identical to normal beta cells.
One pig with a free-fragment autograft is alive and well, with an increase in the
size of its implants, 6 months later. Separation of pancreatic endocrine and exocrine
tissue is not essential for autografting the islets of Langerhans in the pig. The
exocrine tissue apparently can be removed by in vivo mechanisms, and the endocrine
tissue is preserved in free-fragment pancreatic grafts.
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References
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Article info
Footnotes
☆Presented at the First Annual Meeting of the American Society of Transplant Surgeons, Chicago, Ill., May 23, 1975.
Identification
Copyright
© 1976 Published by Elsevier Inc.
