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Abstract
In vitro studies have reported a lack of mesenteric lymph node reactivity against
autochthonous colon carcinoma cells. This study is designed to verify this lack of
reactivity and to correlate its possible relationship with the patients' Dukes classification.
Blastogenic reactivity of peripheral blood lymphocytes (PBLs) and lymph node lymphocytes
(LNLs) to phytohemagglutinin (PHA), allogenic leukocytes in mixed leukocyte culture
(MLC), pokeweed mitogen (PWM), Streptolysin-O (SLO), and to autochthonous tumor cells
(ATCs) was evaluated in 45 patients with colorectal carcinoma at the time of primary
resection of their lesions and was reported as stimulation index (SI). A SI of greater
than 3 was considered to be significant. Five of these patients had Dukes A; 22 had
Dukes B; 12 had Dukes C; and six had Dukes D lesions. Both PBLs and LNLs in Dukes
D reacted vigorously to PHA and PWM. There was a significant difference between PBLs
and LNLs in MLC for both Dukes B and C (p < 0.05), and at all stages LNLs seemed to
be more reactive than PBL to allogeneic leukocytes. PBLs reacted specifically to ATCs
in Dukes B, C, and D (13%, 33%, and 33%, respectively), but failed to react in Dukes
A; whereas LNLs responded to ATCs with SI of greater than 3 only in Dukes B (26%).
A comparably small percentage of PBLs and LNLs responded to SLO in Dukes A, B, and
C, whereas no response to SLO was observed in Dukes D. In conclusion, a dichotomy
of response seemed to be evident in that the nonspecific reactivity to PHA and PWM
seemed to be unrelated to the patients' Dukes classification, but some degree of correlation
seemed to be present between the patient's classification and the reactivity to SLO,
MLC, and ATCs. The specific reactivity to ATCs may be due to the level of antigenicity
and immunogenicity of the tumor cells.
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Article info
Footnotes
☆Presented at the Thirty-fourth Annual Meeting of the Central Surgical Association, Buffalo, N. Y., March 3–5, 1977.
Identification
Copyright
© 1977 Published by Elsevier Inc.
