Advertisement
Surgery
Advanced searchSave search
Central Surgical Association| Volume 82, ISSUE 3, P386-394, September 1977

Intrasplenic islet isografts

DOI:https://doi.org/10.5555/uri:pii:0039606077901507
Intrasplenic islet isografts
Previous ArticleAntibiotic resistance transfer from nonpathogenic to pathogenic bacteria
Next ArticleComplications associated with the use of gastrointestinal stapling devices
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Sufficient numbers of pancreatic islets transplanted into the splenic pulp of streptozotocin-induced diabetic rats can result in complete reversal of hyperglycemia, glycosuria, and polyuria while promoting weight gain. These changes are constant for at least 3 months. Animals transplanted in this way, however, fail to exhibit a normal biphasic insulin response during an intravenous glucose tolerance test. This lack of biphasic response is in marked contrast to that observed in portal-vein-transplanted animals. Basal serum insulin in intrasplenic-transplanted animals is twice that observed in normal animals and portal-vein-transplanted animals which have received the same number of islet isografts. Direct injection of islets into the splenic pulp does not insure that subsequently all islets will remain in the splenic pulp, and, in fact, subsequent splenectomy suggests that in some cases a majority of transplanted tissue lodges in other organs—most likely, the liver.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Surgery
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Ballinger W.F.
        • Lacy P.E.
        Transplantation of intact pancreatic islets in rats.
        Surgery. 1972; 72: 175
        • Colin R.
        • Baillat X.
        L'implantation portale d'ilots pancreatiques exogenes en allogreffes dans le traitment du diabeute experimental.
        Chirurgie. 1974; 100: 571
        • Gray B.N.
        • Watkins Jr., E.
        Prolonged relief from diabetes after syngeneic or allogeneic transplantation of isolated pancreatic islets in rats.
        in: ed. 14. Surg Forum. 25. 1974: 382
        • Griffith R.C.
        • Scharp D.W.
        • Hartman B.K.
        • et al.
        A morphologic study of intrahepatic portal vein islet isografts.
        Diabetes. 1977; 26: 201
        • Kemp C.B.
        • Knight M.J.
        • Scharp D.W.
        • et al.
        Effect of transplantation site on the results of pancreatic islet isografts in diabetic rats.
        Diabetologia. 1973; 9: 486
        • Koncz L.
        • Zimmerman C.E.
        • DeLellis R.A.
        • et al.
        Transplantation of pancreatic islets into the spleen of diabetic rats and subsequent splenectomy.
        Transplantation. 1976; 21: 427
        • Kramp R.C.
        • Cuche R.
        • Renold A.E.
        Subcutaneous, isogeneic transplantation of either duct-ligated pancreas or isolated islets in streptozotocin diabetic mice.
        Endocrinology. 1976; 99: 1161
        • Lacy P.E.
        • Kostianovsky M.
        A method for the isolation of intact islets of Langerhans from the rat pancreas.
        Diabetes. 1967; 16: 35
        • Leichter S.F.
        • Pagliara A.S.
        • Greider M.H.
        • et al.
        Uncontrolled diabetes mellitus in hyperglucagonemia associated with islet cell carcinoma.
        Am J Med. 1975; 58: 285
        • Leonard R.J.
        • Lazarow A.
        • Hegre O.D.
        Pancreatic islet transplantation in the rat.
        Diabetes. 1973; 22: 413
        • Mirkovitch V.
        • Campiche M.
        Successful intrasplenic autotransplantation of pancreatic tissue in totally pancreatectomized dogs.
        Transplantation. 1976; 21: 265
        • Morgan C.R.
        • Lazarow A.
        Immunoassay of insulin to antibody system, possible levels of normal, subdiabetic, and diabetic rats.
        Diabetes. 1963; 12: 115
        • Mullen Y.
        • Brown J.
        • Clark W.R.
        • et al.
        Reversal of experimental diabetes by fetal rat pancreas. Critical procedures for transplantation.
        Transplantation abstracts. 1976; : 34
        • Pipeleers-Marichal M.A.
        • Pipeleers D.G.
        • Cutler J.
        • et al.
        Metabolic and morphologic studies in intraportalislet-transplanted rats.
        Diabetes. 1976; 25: 1041
        • Reckard C.R.
        • Ziegler M.M.
        • Barker C.F.
        Physiologic and immunologic consequences of transplanting isolated pancreatic islets.
        Surgery. 1973; 74: 91
        • Reckard C.R.
        • Ziegler M.M.
        • Naji A.
        • et al.
        Physiologic and immunologic status of long functioning transplanted islets in rats.
        in: ed. 14. Surg Forum. 25. 1974: 373
        • Scharp D.W.
        • Kemp C.B.
        • Knight M.J.
        • et al.
        Use of ficoll in the preparation of viable islets of Langerhans from the rat pancreas.
        Transplantation. 1973; 16: 686
        • Scharp D.W.
        • Murphy J.J.
        • Newton W.T.
        • et al.
        Transplantation of islets of Langerhans in diabetic rhesus monkeys.
        Surgery. 1975; 77: 100
        • Weber C.
        • Weil R.
        • McIntosh R.
        • et al.
        Transplantation of pancreatic islets from neonatal to adult rats.
        Transplantation. 1975; 19: 442
        • Ziegler M.M.
        • Reckard C.R.
        • Barker C.F.
        Long-term metabolic and immunologic considerations in transplantation of pancreatic islets.
        J Surg Res. 1974; 16: 575

      Article info

      Footnotes

      ☆Supported by grants from the Kroc Foundation and the National Institutes of Health (5R01-17188 and AM-18235).

      ☆☆Presented at the Thirty-fourth Annual Meeting of the Central Surgical Association, Buffalo, N. Y., March 3–5 1977.

      Identification

      DOI: https://doi.org/10.5555/uri:pii:0039606077901507

      Copyright

      © 1977 Published by Elsevier Inc.

      ScienceDirect

      Access this article on ScienceDirect
      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties. The content on this site is intended for healthcare professionals.


      RELX