Advertisement
Original communication| Volume 81, ISSUE 4, P469-472, April 1977

Download started.

Ok

Identification of the presence and type of biliary microflora by immediate gram stains

      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Immediate gram stains were performed on gallbladder bile aspirated at the start of an operation for biliary disease in 191 consecutive patients undergoing elective biliary surgery. The results of the gram stains were telephoned to the operating theater within 20 minutes of collection. The over-all accuracy rate of the telephone gram stain reports compared with the subsequent bile cultures was 77 percent. The incidence of false-positive results was 12 percent, and false-negative results were recorded in 7 percent. The organism was identified wrongly by the gram stain in 4 percent of patients. These results have improved, with experience and the over-all accuracy rate of gram stains on bile over the last 6 months have been 87 percent.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Surgery
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Bevan P.G.
        • Williams J.D.
        Rifamide in acute cholecystitis and biliary surgery.
        Br. Med. J. 1971; 3: 284
        • Burke J.F.
        The effective period of antibiotic action in experimental incisions and dermal lesions.
        Surgery. 1961; 50: 161
        • Chetlin S.H.
        • Elleatt D.
        Pre-operative antibiotics in biliary surgery.
        Arch. Surg. 1973; 107: 319
        • Edlund Y.
        • Mollstedt B.O.
        • Ouchterlony O.
        Bacteriological investigations of the biliary system and liver in biliary disease correlated to clinical data and microstructure of the gall bladder and liver.
        Acta Chir. Scand. 1958; 116: 461
        • Engstrom J.
        • Hellstrom K.
        • Hogman L.
        • et al.
        Microorganisms of the liver, biliary tract and duodenal aspirates in biliary diseases.
        Scand. J. Gastroenterol. 1971; 6 (1971): 177
        • Keighley M.R.B.
        Micro-organisms in the bile: A preventable cause of sepsis after biliary surgery.
        in: M. S. thesis. University of London, London1976
        • Keighley M.R.B.
        • Flinn R.
        • Alexander-Williams J.
        Multivariate analysis of clinical and operative findings associated with biliary sepsis.
        Br. J. Surg. 1976; 63: 528
        • Keighley M.R.B.
        • Lister D.M.
        • Jacobs S.I.
        • et al.
        Hazards of surgical treatment due to micro-organisms in the bile.
        Surgery. 1974; 75: 578
        • Keighley M.R.B.
        • Drysdale R.B.
        • Quoraishi A.H.
        • et al.
        Antibiotic treatment of biliary sepsis.
        Surg. Clin. North Am. 1975; 55: 1379
        • Scott A.J.
        Bacteria and disease of the biliary tract.
        Gut. 1971; 12: 487