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Original communication| Volume 83, ISSUE 2, P188-193, February 1978

Insulin-releasing activity and successful transplantation of pancreatic islets preserved by tissue culture

DOI:https://doi.org/10.5555/uri:pii:0039606078902775
Insulin-releasing activity and successful transplantation of pancreatic islets preserved by tissue culture
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      Abstract

      Preserving pancreatic islets for 7 days by a making use of the organ culture, we studied the insulin-releasing activity at the time of administration of glucose and various digestive tract hormones for the purpose of clarifying the function of preserved pancreatic islets. Furthermore, we transplanted pancreatic islets preserved for 3 to 5 days into the portal vein of rats with streptozotocin-induced diabetes and reached the following conclusions: (1). The islets of Langerhans of the pancreas responded well to glucose up to the seventh day of preservation and showed patterns similar to those of fresh pancreatic islets with respect to both the dose response and the time response. (2). Preserved pancreatic islets of the pancreas had insulin-releasing activity almost equal to that of fresh pancreatic islets against stimulation by glucagon, tolbutamide, and various digestive tract hormones. (3). Rats with streptozotocin-induced diabetes showed a marked improvement in blood glucose and urine glucose following transplantation of preserved pancreatic islets into the portal vein, and this effectiveness persisted for 6 to 8 weeks.
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      References

        • Ballinger W.F.
        • Lacy P.E.
        Transplantation of intact pancreatic islets in rats.
        Surgery. 1972; 72: 175
        • Ballinger W.F.
        • Lacy P.E.
        Effect of transplantation site on the results of pancreatic islets isografts in diabetic rats.
        Diabetologia. 1973; 9: 486
        • Bergan J.J.
        • Hoehn J.G.
        • Porter N.
        • et al.
        Total pancreatic allografts in pancreatectomized dogs.
        Arch Surg. 1965; 90: 521
        • Ferguson J.
        • Scothorne R.J.
        Transplantation of the endocrine pancreas in guinea pigs.
        Br J Surg. 1972; 59: 316
        • Idezuki Y.
        • Feemster J.A.
        • Dietzman R.H.
        • et al.
        Experimental pancreaticduodenal preservation and transplantation.
        Surg Gynecol Obstet. 1968; 126: 1002
        • Lacy P.E.
        • Kastianovsky M.
        Method for the isolation of intact islets of Langerhans from the rat pancreas.
        Diabetes. 1967; 16: 35
        • Mauer S.M.
        • Sutherland D.E.R.
        • Steffes M.W.
        • et al.
        Pancreatic islet transplantation—effects on the glomerular lesions of experimental diabetes in the rat.
        Diabetes. 1974; 23: 748
        • Moskalewski S.
        Isolation and culture of the islets of Langerhans of the guinea pig.
        Gen Comp Endocrinol. 1965; 5: 342
        • Reckard C.R.
        • Barker C.F.
        Transplantation of isolated pancreatic islets across strong and weak histocompatibility barriers.
        Transplant Proc. 1973; 5: 761
        • Reckard C.R.
        • Ziegler M.M.
        • Barker C.F.
        Physiological and immunological consequences of transplanting isolated pancreatic islets.
        Surgery. 1973; 74: 91
        • Steffes M.W.
        • Sutherland D.E.R.
        • Mauer S.M.
        • et al.
        Islet transplantation in diabetic rats receiving corticosteroids.
        Transplantation. 1975; 19: 449
        • Steffes M.W.
        • Sutherland D.E.R.
        • Mauer S.M.
        • et al.
        Plasma insulin and glucose levels in diabetic rats prior to and following islet transplantation.
        J Lab Clin Med. 1975; 85: 75
        • Weil III, R.
        • Nozawa M.
        • Koss M.
        • et al.
        Pancreatic transplantation in diabetic rats: Renal function, morphology, ultrastructure, and immunohistology.
        Surgery. 1975; 78: 142

      Article info

      Publication history

      Accepted: April 12, 1977

      Identification

      DOI: https://doi.org/10.5555/uri:pii:0039606078902775

      Copyright

      © 1978 Published by Elsevier Inc.

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