Original communication| Volume 83, ISSUE 2, P206-213, February 1978

Effect of a synthetic analogue of PGE2 on exocrine and endocrine pancreatic function in the rat

      This paper is only available as a PDF. To read, Please Download here.


      The effect of 16,16-dimethyl PGE2-methyl-ester (di-M-PGE2 a long-acting synthetic analogue of PGE2) on exocrine and endocrine pancreatic secretion was studied in rats with chronic pancreatic fistulas. Under basal conditions as well as after stimulation with secretin and OP-CCK, pancreatic exocrine secretion was inhibited markedly by intravenous injection of di-M-PGE2 at 10 μg/kg and 100μg/kg. Secretory volumes and bicarbonate and protein outputs decreased within 10 minutes after the administration of di-M-PGE2, and this inhibitory effect persisted throughout the following 40 minutes. The smaller dose of di-M-PGE2 (10 μg/kg) inhibited the volume of pancreatic secretion by an average of 47.7% and decreased bicarbonate and protein output by 41.1% and 70.5%, respectively. The larger dose (100 μg/kg) caused a mean 48.3% inhibition of pancreatic secretory volume and decreased bicarbonate and protein outputs by 41.7% and 64.5%, respectively. Plasma insulin levels were lowered markedly after injection of di-M-PGE2 under basal conditions (mean inhibition 63.1% by PG-10 and 55.3% by PG-100) as well as after secretin stimulation (mean inhibition 89.5% by PG-10 and 82.4% by PG-100). These observations document that di-M-PGE2 is a potent inhibitor of pancreatic exocrine and endocrine function in the unanesthetized rat. In these actions the PGE2-analogue antagonized the stimulatory effects of both secretin and OP-CCK.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Surgery
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Bressler R.
        • Vargas-Cordon M.
        • Lebovitz H.E.
        Tranylcypromine: A potent insulin secretagogue and hypoglycemic agent.
        Diabetes. 1968; 17: 617
        • Case R.M.
        • Scratcherd T.
        Prostaglandin action on pancreatic blood flow and on electrolyte and enzyme secretion by exocrine pancreas in vivo and in vitro.
        J Physiol. 1972; 226: 393
        • Enk B.
        • Lund B.
        • Schmidt A.
        • et al.
        Secretin-induced insulin response.
        Acta Endocrinol. 1976; 82: 306
        • Gabryelewicz A.
        • Szalaj W.
        • Kinalska I.
        • et al.
        The effect of prostaglandin E1 on exocrine pancreatic secretion (in vivo and in vitro).
        Pol J Pharmacol Pharm. 1974; 26: 263
        • Johnson D.G.
        • Fujimoto W.Y.
        • Williams R.H.
        Enhanced release of insulin by prostaglandins in isolated pancreatic islets.
        Diabetes. 1973; 22: 658
        • Lefebvre P.J.
        • Luyckx A.S.
        Stimulation of insulin secretion after prostaglandin PGE2 in the anesthetized dog.
        Biochem Pharmacol. 1973; 22: 1773
        • Lowry O.H.
        • Rosebrough N.J.
        • Farr A.L.
        • et al.
        Protein measurements with the Folin phenol reagent.
        J Biol Chem. 1951; 193: 265
        • Main J.H.M.
        Effects of prostaglandin E2 (PGE2) on the output of histamine and acid in rat gastric secretion induced by pentagastrin or histamine.
        Br J Pharmacol Soc. 1969; 36: 214P
        • Morgan C.R.
        • Lazarow A.
        Immunoassay of insulin: Two antibody system. Plasma insulin levels of normal, subdiabetic, and diabetic rats.
        Diabetes. 1963; 12: 115
        • Pagliara A.S.
        • Stillings S.N.
        • Hover B.
        • et al.
        Glucose modulation of amino acid-induced glucagon and insulin release in the isolated perfused rat pancreas.
        J Clin Invest. 1974; 54: 819
        • Pedrazzoli S.
        • Dodi G.
        • Lise M.
        Technique for collection of pancreatic juice from rats.
        Rendic. Gastroenterol. 1975; 7: 31
        • Pek S.
        • Tai T.Y.
        • Elster A.
        • et al.
        Stimulation by prostaglandin E2 of glucagon and insulin release from isolated rat pancreas.
        Prostaglandins. 1975; 10: 493
        • Robert A.
        Effect of prostaglandins on the stomach and the intestine.
        Prostaglandins. 1974; 6: 523
        • Robert A.
        • Nezamis J.E.
        • Phillips J.P.
        Effect of prostaglandin E1 on gastric secretion and ulcer formation in the rat.
        Gastroenterology. 1968; 55: 481
        • Robertson R.P.
        • Gavareski D.J.
        • Porte D.
        • et al.
        Prostaglandin (PG)E1: inhibition of glucose-stimulated insulin secretion in the intact dog.
        Clin Res. 1973; 21: 635
        • Rosenberg V.
        • Biezunski D.
        • Gonda M.
        • et al.
        Influence of a synthetic prostaglandin analog on pancreatic secretion.
        Surgery. 1976; 79: 509
        • Rossini A.A.
        • Lee J.B.
        • Frawley T.F.
        An unpredictable lack of effect of prostaglandins on insulin release in isolated rat islets.
        Diabetes. 1971; 20 (Suppl): 374
        • Rudick J.
        • Gonda M.
        • Dreiling D.A.
        • et al.
        Effects of prostaglandin E1 on pancreatic exocrine function.
        Gastroenterology. 1971; 60: 272
        • Saunder R.N.
        • Moser C.A.
        Changes in vascular resistance induced by prostaglandins E2 and F2α in the isolated rat pancreas.
        Arch Int Pharmacodyn Ther. 1972; 197: 86
        • Spellacy W.N.
        • Buhi W.C.
        • Holsinger K.K.
        The effect of prostaglandin F2α and E2 on blood glucose and plasma insulin levels during pregnancy.
        Am J. Obstet. Gynecol. 1971; 111: 239