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Abstract
Experiments were designed to test the hypothesis that prolonged culture of endocrine
pancreas might result in a decrease in immunogenicity. Eighteen-day gestation fetal
rat pancreas was maintained in organ culture (medium RPMI 1640 + 10% fetal calf serum
for 14 to 21 days and then transplanted under the kidney capsule of streptozotocin-induced
diabetic rats. Eight to 10 donor pancreata were used per recipient. Each recipient
was treated with 1 to 3 units of Ultralente Insulin per day for the first 8 days after
transplant. Following isotransplantation (Lewis to Lewis) of pancreata after 21 days
in culture, there was a complete correction of the diabetic state 3 weeks after transplantation.
At 8 to 9 weeks after transplantation, the grafts were composed of solid islands of
B cells surrounded by adipose tissue at the site of implantation over the kidney cortex.
Allotransplantation of (DA × Lewis) Fl fetal pancreata to Lewis recipients after 21
days' culture failed to show any significant prolongation of survival over noncultured
allografts (rejection time, 10 days). At 8 weeks after transplantation, the only evidence
of the implanted cultured pancreas allograft was a slight scar on the outer surface
of the kidney cortex. Histologically there was no trace of pancreatic tissue, which
was replaced by fibrous tissue and a mononuclear cell infiltrate. We conclude that
in vitro culture for a period up to 21 days does not prevent rejection of fetal pancreatic
allografts in this model.
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Article info
Publication history
Accepted:
March 28,
1979
Footnotes
☆Supported by grants from the Medical Research Council, the British Diabetic Association, and the Wellcome Foundation.
Identification
Copyright
© 1980 Published by Elsevier Inc.
