Original communication| Volume 87, ISSUE 2, P157-163, February 1980

Download started.


Rejection of organ-cultured allogeneic fetal rat pancreas

      This paper is only available as a PDF. To read, Please Download here.


      Experiments were designed to test the hypothesis that prolonged culture of endocrine pancreas might result in a decrease in immunogenicity. Eighteen-day gestation fetal rat pancreas was maintained in organ culture (medium RPMI 1640 + 10% fetal calf serum for 14 to 21 days and then transplanted under the kidney capsule of streptozotocin-induced diabetic rats. Eight to 10 donor pancreata were used per recipient. Each recipient was treated with 1 to 3 units of Ultralente Insulin per day for the first 8 days after transplant. Following isotransplantation (Lewis to Lewis) of pancreata after 21 days in culture, there was a complete correction of the diabetic state 3 weeks after transplantation. At 8 to 9 weeks after transplantation, the grafts were composed of solid islands of B cells surrounded by adipose tissue at the site of implantation over the kidney cortex. Allotransplantation of (DA × Lewis) Fl fetal pancreata to Lewis recipients after 21 days' culture failed to show any significant prolongation of survival over noncultured allografts (rejection time, 10 days). At 8 weeks after transplantation, the only evidence of the implanted cultured pancreas allograft was a slight scar on the outer surface of the kidney cortex. Histologically there was no trace of pancreatic tissue, which was replaced by fibrous tissue and a mononuclear cell infiltrate. We conclude that in vitro culture for a period up to 21 days does not prevent rejection of fetal pancreatic allografts in this model.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Surgery
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Andersson A
        • Buschard K
        Culture of isolated pancreatic islets: Its applications for transplantation purposes.
        Trans Am Soc Artif Intern Organs. 1977; 23: 342
        • Barker CF
        Transplantation of the islets of Langerhans and the histocompatibility of endocrine tissue.
        Diabetes. 1975; 24: 766
        • Berjon JJ
        • Meunier JM
        Outcome of fetal pancreas grafts implanted in mature animals after organ culture on artificial medium.
        Bordeaux Med. 1972; 5: 2723
        • Brown J
        • Clark WR
        • Molnar IG
        • et al.
        Fetal pancreas transplantation for reversal of streptozotocin induced diabetes in rats.
        Diabetes. 1976; 25: 56
        • Finch DR
        • Morris PJ
        The effect of increasing islet numbers on survival of pancreatic islet allografts in immunosuppressed diabetic rats.
        Transplantation. 1977; 23: 104
        • Hegre OD
        • Leonard RJ
        • Erlandsen SL
        • et al.
        Transplantation of islet tissue in the rat.
        Acta Endocrinol. 1976; 205 (Suppl):: 257
        • Hickson J
        The patchwork mouse.
        Anchor Press/Doubleday & Co., Inc, Garden City, NY1976
        • Jacobs BB
        Ovarian allograft survival. Prolongation after passenge in vitro.
        Transplantation. 1974; 18: 454
        • Jonasson O
        Culture and transplantation of fetal primate pancreas.
        Transplant Proc. 1979; 11: 1159
        • Kedinger M
        • Haffen K
        • Grenier J
        • et al.
        In vitro culture reduces immunogenicity of pancreatic endocrine islets.
        Nature. 1977; 270: 736
        • Lafferty KJ
        • Cooley MA
        • Woolnough J
        • et al.
        Thyroid allograft immunogenicity is reduced after a period in organ culture.
        Science. 1975; 188: 259
        • Lafferty KJ
        • Bootes A
        • Dart G
        • et al.
        Effect of organ culture on the survival of thyroid allografts in mice.
        Transplantation. 1976; 22: 138
        • Leuker DC
        • Sharpton TR
        Survival of ovarian allografts following maintenance in organ culture.
        Transplantation. 1974; 18: 457
      1. Mandel TE: Organ culture and transplantation of foetal mouse pancreatic islets. Transplant Proc (in press)

        • Ninnemann JL
        • Good RA
        Allogeneic transplantation of organ cultures without immunosuppression. An evaluation using adult mouse skin.
        Transplantation. 1974; 18: 1
        • Raaf JH
        • Van Pilsum JF
        • Good RA
        Fresh and cultured thyroid gland: Survival and function after implantation.
        Ann Surg. 1976; 183: 146
        • Seltzer HS
        Complete reversal of alloxan diabetes in rats by islet transplants from unrelated donors.
        Univ Mich Med Cen J. 1976; 42: 12
        • Sollinger HW
        • Burkholder PM
        • Rasmus WR
        • et al.
        Prolonged survival of xenografts after organ culture.
        Surgery. 1977; 81: 74
        • Starling JR
        • Fidler R
        • Corry RJ
        Prolongation of survival of rat parathyroid allografts by enhancing serum and tissue culture.
        Surgery. 1977; 81: 668
        • Summerlin WT
        Allogeneic transplantation of organ cultures of adult human skin.
        Clin Immunol Immunopathol. 1973; 1: 372
        • Summerlin WT
        • Broutbar C
        • Foanes RB
        • et al.
        Acceptance of phenotypically differing cultured skin in man and mice.
        Transplant Proc. 1973; 5: 707