Original communication| Volume 87, ISSUE 2, P170-176, February 1980

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Antigen depressant effect of glutaraldehyde for aortic heterografts with a valve, with special reference to a concentration right fit for the preservation of grafts

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      A suitable concentration of glutaraldehyde for preservation of aortic heterografts with a valve was investigated from the view points of immunologic, histological, and hemodynamic studies. A heterograft model was prepared using dogs and raccoon dogs in such a way that aortas with valves of raccoon dogs were implanted into the descending aortas of dogs. A pressure study at 2 to 12 months after implantation demonstrated the following results: there was no pressure gradient between the graft and recipient aorta in the fresh graft group, there was a slight pressure gradient of 11 ± 3 and 10 ± 3 mm Hg m the 0.05% and 0.15% glutaraldehyde-preserved groups, respectively, and a marked pressure gradient of 49 ± 2 mm Hg in the 0.3% glutaraldehyde group. Angiography showed aneurysmal dilatation of implanted grafts in a fresh graft group, no demonstrable deformity in the 0.05% and 0.15% preserved grafts, and a marked stenosis and shrinkage of grafts in the 0.3% group. These changes were substantiated from macroscopic and histological observation. Immunologic analysis of recipient dog sera using techniques for antiraccoon-dog hemagglutinin, hemolysin, and lymphocytotoxin revealed an obvious antigen depressant effect of glutaraldehyde, even in cases in which highly diluted solution (0.0.5%) was used. These findings may lead us to conclude that a low-concentration solution of glutaraldehyde (0.15%, or less) is the choice for treatment of heterografts.
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