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Abstract
The effect of hemoglobin on the ability of polymorphonuclear granulocytes (PMNs) to
phagocytize and kill opsonized E. coli was measured. Results show that the addition
of hemoglobin in a concentration of 0.1% inhibitis phagocytotic activity of PMNs from
68% to 13% after 30 minutes and from 73% to 45% after 60 minutes. The rate of intracellular
killing after the addition of hemoglobin in the concentration of 0.5% declined from
63% to 21%. Though these experiments do not allow any conclusions as to the exact
mechanism of action of hemoglobin in inhibition of phagocytosis, it seems likely from
other data that the inhibitory activity resides in the heme part of the molecule.
We conclude that hemoglobin inhibits phagocytotic activity and the ability to kill
ingested bacteria of human polymorphonuclear granulocytes. This provides an additional
mechanism for the adjuvant action of hemoglobin in intraperitoneal infections and
supports the theory that hemoglobin acts directly on the granulocyte to impair the
essential host defenses.
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Article info
Publication history
Accepted:
October 17,
1979
Footnotes
☆Supported by U.S. Public Health Service grant No. AI 14032 and by National Institutes of Health, Bethesda, Md., fellowship No. IF32 HL05378, (T.H.).
Identification
Copyright
© 1980 Published by Elsevier Inc.
