This paper is only available as a PDF. To read, Please Download here.
The effect of hemoglobin on the ability of polymorphonuclear granulocytes (PMNs) to phagocytize and kill opsonized E. coli was measured. Results show that the addition of hemoglobin in a concentration of 0.1% inhibitis phagocytotic activity of PMNs from 68% to 13% after 30 minutes and from 73% to 45% after 60 minutes. The rate of intracellular killing after the addition of hemoglobin in the concentration of 0.5% declined from 63% to 21%. Though these experiments do not allow any conclusions as to the exact mechanism of action of hemoglobin in inhibition of phagocytosis, it seems likely from other data that the inhibitory activity resides in the heme part of the molecule. We conclude that hemoglobin inhibits phagocytotic activity and the ability to kill ingested bacteria of human polymorphonuclear granulocytes. This provides an additional mechanism for the adjuvant action of hemoglobin in intraperitoneal infections and supports the theory that hemoglobin acts directly on the granulocyte to impair the essential host defenses.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Surgery
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- The pathogenesis of strangulation obstruction. Effect of hemoglobin on bactericidal capacity of human leukocytes.J Surg Res. 1965; 5: 262
- Enhancement of Escherichia coli infection and endotoxic activity by hemoglobin and ferric ammonium citrate.Surgery. 1970; 68: 350
- Determinants of infection in the peritoneal cavity. III. The action of selected inhibitors on the fate of Staphylococcus aureus in the mouse.Yale J Biol Med. 1962–1963; 35: 48
- A possible toxic factor in abdominal injury.J Trauma. 1962; 2: 291
- A toxic factor in abdominal injury. II. The role of the red cell component.J Trauma. 1964; 4: 84
- Inhibition of thymidine uptake by staphylococci, a new method for the investigation of phagocytosis.J Immunol Meth. 1976; 11: 345
- Mechanisms of the adjuvant action of hemoglobin in experimental peritonitis. II. Influence of hemoglobin on human leukocyte chemotaxis in vitro.J Surg Res. 1977; 22: 174
- Mechanisms of the adjuvant effect of hemoglobin in experimental peritonitis. I. In vivo inhibition of peritoneal leukocytosis.Surgery. 1978; 83: 223
- The antibacterial activity of hemoglobin.J Exp Med. 1958; 107: 167
- Metabolic basis of phagocytotic activity.Physiol Rev. 1962; 42: 143
- The role of the red cell in subcutaneous infection.J Trauma. 1965; 5: 85
- Mechanisms of the adjuvant effect of hemoglobin in experimental peritonitis. V. The Significance of the coordinated iron component.Surgery. 1979; 86: 41
- Particle uptake by polymorphonuclear leukocytes and Ehrlich ascites-carcinoma cells.Biochem J. 1963; 89: 150
- The biochemical basis for phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes.J Biol Chem. 1959; 234: 1355
- Pathogenesis of peritonitis. III. Local adjuvant action of hemoglobin in experimental E. coli peritonitis.Surgery. 1968; 63: 810
- Quantitative studies of phagocytosis by polymorphonuclear leukocytes: use of emulsions to measure the initial rate of phagocytosis.J Clin Invest. 1972; 51: 615
- Inhibition of macrophage mediated tumor killing by phagocytized red blood cells and Candida albicans.J Reticuloendothel Soc. 1976; 20: 37a
- Antibacterial activity of hydrolyzed red blood cells in vitro.in: ed 2. Proc Soc Exp Biol Med. 74. 1950: 346
- The effects of the morphine analogue levophanol on leukocytes metabolic effects at rest and during phagocytosis.J Clin Invest. 1971; 50: 1091
Accepted: October 17, 1979
☆Supported by U.S. Public Health Service grant No. AI 14032 and by National Institutes of Health, Bethesda, Md., fellowship No. IF32 HL05378, (T.H.).
© 1980 Published by Elsevier Inc.