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Abstract
We report here the first development of a continuous cell line in tissue culture of
an animal pancreatic duct adenocarcinoma that is histologically similar to human pancreatic
duct adenocarcinoma. A primary pancreatic duct adenocarcinoma, induced in a male Syrian
golden hamster after 23 weeks of weekly subcutaneous injections of N-nitrobis(2-hydroxypropyl)amine,
was minced and injected subcutaneously into three hamsters. After 8 weeks, a single
tumor was apparent. Subsequent passages of fragments into the cheek pouches were performed
at 3- to 4-week intervals. After five passages, minced fragments of a tumor were placed
in tissue culture. Colonies appeared by 7 days; an epitheloid cell line, without fibroblasts,
was established by 60 days. Single-cell suspensions, injected into hamster cheek pouches
or subcutaneously, produced tumors in a dose-dependent fashion. Spent culture medium
of tissue culture cells and saline extracts of freshly excised tumors contained pancreatic
oncofetal antigen-like activity.
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Article info
Publication history
Accepted:
March 4,
1982
Identification
Copyright
© 1982 Published by Elsevier Inc.
