This paper is only available as a PDF. To read, Please Download here.
Pancreatic secretion of enzymes and gallbladder contraction in response to intestinal stimulants are thought to be mediated through the vagus nerve and by means of release of cholecystokinin (CCK). The effect of truncal vagotomy on the release of CCK, pancreatic protein secretion, and gallbladder pressure (all stimulated by intraduodenal instillation of oleate) was studied in five dogs. Each dog was prepared with chronic pancreatic and gastric fistulas and catheter cholecystostomies. Simultaneous measurements were made of plasma CCK (by radioimmunoassay), pancreatic protein secretion, and gallbladder pressure (by perfused catheter technique) before and during intraduodenal administration of oleate. Before truncal vagotomy, intraduodenal oleate caused increases in plasma CCK (from 82 ± 6 to 208 ± 32 pg/ml), pancreatic protein secretion (from 83 ± 8 to 165 ± 15 mg/15 min), and gallbladder pressure (from 77 ± 2 to 27 ± 2 cm H2O) (all measured from basal state to 120 minutes). Truncal vagotomy caused a 45% decrease in the output of pancreatic protein in response to oleate and completely abolished the increase in gallbladder pressure, but it caused no change in release of CCK. The correlations between plasma CCK and pancreatic protein secretion before truncal vagotomy (r = 0.86) and after truncal vagotomy (r = 0.77) were highly significant. The correlation between plasma CCK and gallbladder pressure was highly significant before (r = 0.91) but not after (r = 0.42) truncal vagotomy. This study demonstrates that truncal vagotomy inhibits pancreatic protein secretion and gallbladder pressure in response to fat but does not interfere with release of CCK. The effects may be due to interruption of vagus-mediated reflexes between the intestine and the pancreas and gallbladder. The good correlation between plasma concentrations of CCK and both pancreatic protein secretion and gallbladder pressure provides evidence that the radioimmunoassay measures biologically active CCK.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Surgery
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- The effects of vagotomy upon biliary function in dogs.J Surg Res. 1970; 10: 209-212
- Studies with cholecystokinin. II. Cholecystokinetic potency of porcine gastrins I and II and related peptides in three systems.Endocrinology. 1969; 84: 1277-1281
- Effect of extragastric and truncal vagotomy on pancreatic secretion in the dog.Am J Physiol. 1975; 228: 1172-1177
- Evidence for a pyloro-cholecystic reflex for gallbladder contraction.Ann Surg. 1979; 190: 170-175
- Changes in canine bile-flow and composition after vagotomy.Br J Surg. 1969; 56: 103-106
- Preparation of 131I labelled human growth hormone to high specific radioactivity.Biochem J. 1963; 89: 114-123
- Effect of vagal stimulation on pancreatic secretion and on blood levels of gastrin, cholecystokinin, secretin, vasoactive intestinal peptide, and somatostatin.Surgery. 1979; 86: 329-336
- Human gallbladder function after selective gastric and total abdominal vagotomy.Acta Chir Scand. 1969; 135: 625-633
- Increased release of cholecystokinin after pancreatic duct ligation.Surgery. 1982; 91: 467-473
- The effect of vagotomy on motor function of the gallbladder.Surgery. 1971; 70: 616-621
- Effect of vagotomy on hormones stimulating pancreatic secretion.Arch Surg. 1974; 108: 704-708
- Effect of vagotomy on pancreatic secretion evoked by endogenous and exogenous cholecystokinin and caerulein.Gastroenterology. 1972; 63: 273-278
- Infusion of pure cholecystokinin in man. Correlation between plasma concentrations of CCK and gallbladder size.Gastroenterology. 1982; 83: 256-261
- Effect of alcohol on the release of secretin and pancreatic secretion.Surgery. 1977; 81: 661-667
- Release of pancreatic polypeptide in humans by infusion of cholecystokinin.Gastroenterology. 1980; 79: 817-822
- Protein measurement with the Folin reagent.J Biol Chem. 1951; 193: 265-275
- Release of secretin and cholecystokinin.in: Thompson JC Gastrointestinal hormones. University of Texas Press, Austin1975: 475-489
- Manometric characteristics of the extrahepatic biliary tract in dogs.Dig Dis Sci. 1981; 26: 417-422
- Gallbladder volume and contractility after truncal, selective and highly selective (parietal-cell) vagotomy in man.Ann Surg. 1973; 178: 581-586
- Secretin, cholecystokinin, and newer gastrointestinal hormones.N Engl J Med. 1976; 294: 1093-1101
- Secretin, cholecystokinin, and newer gastrointestinal hormones.N Engl J Med. 1976; 294: 1157-1164
- Effect of atropine on secretion from intact and transplanted pancreas in dog.Am J Physiol. 1980; 238: G18-G22
- Latency of pancreatic enzyme response to intraduodenal stimulants.Am J Physiol. 1980; 238: G23-G29
- Vagal control of pancreatic exocrine secretion.in: Brooks FP Evers PW Nerves and the gut. Charles B Slack, Thorofare, NJ1977: 119-132
- Gastrin-related peptides as stimulants of pancreatic and gastric secretion.Am J Physiol. 1969; 217: 262-266
- Pancreatic secretion after selective and truncal vagotomy in the dog.Br J Surg. 1971; 58: 839-844
- An improved cannula for gastric and intestinal fistulas.in: Proc Soc Exp Biol Med. 46. 1941: 260-261
- Effect of secretin on circulating gastrin.Ann Surg. 1972; 176: 384-393
- Release of cholecystokinin in man: Correlation of blood levels with gallbladder contraction.Ann Surg. 1981; 194: 321-327
Accepted: September 22, 1982
☆Supported by National Institutes of Health grant AM 15241 and Moody Foundation grant 81-57.
© 1983 Published by Elsevier Inc.