Advertisement

Action of carnosine and β-alanine on wound healing

      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      In rats treat-given hydrocortisone to suppress healing, tensile strength of the skin at the site of an incision wound was significantly higher in rats locally treated with carnosine than in untreated animals. Similar effects on the tensile strength of the skin were observed by the administration of β-alanine and histidine, but not of β-alanine alone. Exogenous carnosine was degraded in the body by carnosinase and histidine decarboxylase to yield histamine. Since β-alanine, the other degradation product of carnosine, was found to stimulate the biosynthesis of nucleic acids and collagen, histamine derived from carnosine is considered to have enhanced the process of wound healing by stimulating effusion at the initial stage of inflammation. Thus, the enhancement by carnosine of wound healing may be ascribed to stimulation of early effusion by histamine and of collagen biosynthesis by β-alanine. The wound-healing effects of carnosine were further demonstrated by the observation that carnosine significantly increased granulation suppressed by cortisone, mytomycin C, 5-fluorouracil, and bleomycin.

      Resumen

      Se observó que la fuerza de tensión de la piel en el sitio de una incisión hecha en ratas, que habian recibido hidrocortisona para suprimir la cicatrización, fue significativamente más grande en ratas que habian sido tratadas localmente con carnosina que los animales no tratados. Con la administración de β-alanina e histidina se observaron efectos semejantes sobre la fuerza de tensión. Estos efectos no se observaron si se utilizó β-alanina en forma aislada. La carnosina exógena se degrada en el cuerpo por la carnosinasa y HDC para producir histamina, ya que se ha observado que la β-alanina, que es un producto de degradación de la carnosina, estimula la biosíntesis de ácidos nucléicos y colágeno. Nosotros consideramos que la histamina derivada de la carnosina estimula el proceso de cicatrización produciendo una efusión durante las etapas iniciales de la inflamación. De esta manera, el efecto benéfico que tiene la carnosina sobre la cicatrización, se debe a la producción de un derrame inicial producido por la histamina, y a una biosíntesis de colágeno inducido por β-alanina. Los efectos benéficos de la carnosina sobre la cicatrización, fueron observados también en el aumento de tejido de granulación que habia sido suprimido por la cortisona, MMC, 5-FU y bleomisina.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Surgery
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Gulewitsch W
        • Amiradzibi S
        Uber des Carnosin, eine neue organische Base des Fleischextraktes.
        Ber Drch Chem Ges. 1900; 33: 1902-1905
        • Nagai K
        • Murakami H
        • Sano A
        • Kabutake H
        Die physiologische Beteutung von Carnosin und Homocarnosin.
        Arzneim Forsch (Drug Res). 1970; 20: 1876-1878
        • Henmi I
        • Katayama K
        • Kono M
        Heilende Wirkung von l-Carnosin auf Operationswunde bei Apikoektomie und Gingivektomie.
        J Nihon Univ Sch Dent. 1977; 19: 16-24
        • Yamane T
        • Kishi K
        • Tajima T
        • Agariguchi H
        • Uwazumi K
        • Katayama K
        Heilende Wirkung von l-Carnosin auf Operationswunde und Geschwür bei zahnchirurgische Implantation.
        J Nihon Univ Sch Dent. 1977; 19: 70-92
        • Murase M
        • Takai H
        • Hinata H
        • Uchida Y
        • Ohme A
        • Hamada N
        • Okano H
        • Iida T
        • Takahashi S
        • Sato S
        • Suzuki T
        • Nagai K
        Clinical studies on l-carnosine (N-2-M) dental cone by the double blind test.
        Jpn J Oral Surg. 1974; 20: 173-183
        • Shimanaka S
        Spontaneous healing: Promotive effect of carnosine (β-alanyl-l-histidine) on the cervical erosion. Proceeding.
        in: IUPHAR Ninth International Congress of Pharmacology. London. 1984: 626 (Book of abstracts)
        • Nagai K
        A medicine for treatment of cervical erosion.
        U.S.P. 4446. 1984; : 149
        • Fisher DE
        • Amend JF
        • Strumeyer DH
        • Fisher H
        A role for carnosine and anserine in histamine metabolism of the traumatized rat.
        in: ed 1. Proc Soc Exp Biol Med. 158. 1978: 402-405
        • Fitzpatrick DW
        • Fisher H
        Carnosine, histidine, and wound healing.
        Surgery. 1982; 91: 56-60
        • Vizioli MR
        • Almeida OP
        Effects of carnosine on the development of rat sponge-induced granulation. I. General morphology and glycosaminoglycans histophotometry.
        Cell Mol Biol. 1978; 23: 267-273
        • Vizioli MR
        • Blumen G
        • Almeida OP
        • Munhoz COG
        Effects of carnosine on the development of rat sponge-induced granulation. II. Histoautoradiographic observations on collagen biosynthesis.
        Cell Mol Biol. 1983; 29: 1-9
        • Hanson HT
        • Smith EL
        Carnosinase; An enzyme of swine kidney.
        J Biol Chem. 1949; 179: 789-801
        • Watanabe T
        • Taguchi Y
        • Wada H
        Metabolism of histamine and related enzymes.
        Tanpakushitsu Kakusan Koso. 1981; 26: 1447-1461
        • Tanaka A
        • Kobayashi F
        • Miyake T
        A new anti-inflammatory activity test for corticosteroids (The formalin filter paper pellet method).
        Endocrinol Jpn. 1960; 7: 364-375
        • Volkin E
        • Cohn WE
        Methods of biochemical analysis. 1954; 1: 287
        • Burton K
        Biochem J. 1956; 62: 315
        • Brown AH
        Arch Biochem. 1946; 11: 269
        • Kahlson G
        • Rosengren E
        1971. Biogenesis and physiology of histamine.
        Edward Arnold Ltd, London1971
        • Ikenoya H
        • Suda T
        • Kawasaki K
        • Nagai K
        Acceleration of wound healing by local administration of carnosine with cortisone: Conversion of anti-granulation effects of cortisone to pro-granulation effects.
        Saishin Igaku. 1984; 39: 1957-1960