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Abstract
The actions of serotonin and substance P have been examined with use of an isolated,
vascularly perfused rabbit ileal preparation. The vascular perfusate was composed
of a modified Krebs' buffer solution that contained washed human red blood cells (hematocrit,
15% to 20%) and 3% albumin, with no added hormones or peptides. Ileal blood flow was
held constant at 49.3 ± 3.1 ml/min per 100 gm wet weight of intestine. Net intestinal
water and electrolyte fluxes were calculated by means of an isosmotic buffer that
contained carbon-14 polyethylene glycol as a nonabsorbable volume marker. Viability
of this isolated perfused ileal preparation was confirmed on the basis of light microscopy,
oxygen consumption, and transmucosal potential difference measurements. Control experiments,
without exogenous hormone infusion, resulted in a stable preparation with a basal
secretory state. Intra-arterial serotonin at 2.5 μg/min (n = 10) significantly stimulated
secretion of H20, Na+, and Cl− (p < 0.01). Intra-arterial substance P at 2.5 × 10−1 μg/min (n = 7) significantly increased the secretion of H20, Na+, and Cl−1 (p < 0.02). The dose of serotonin was designed to yield serotonin levels that resembled
those found circulating in patients with carcinoid syndrome. These data indicate that
serotonin and substance P are potent secretagogues in a mammalian system, independent
of their effect on mesenteric blood flow and in the absence of extra-intestinal hormonal
and neural influences.
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Article info
Publication history
Accepted:
May 5,
1988
Footnotes
☆Presented in part at the Surgical Forum, San Francisco, Calif., October 1987.
Identification
Copyright
© 1989 Published by Elsevier Inc.
