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Abstract
Four different intravenous catheter materials, brands Teflon, Silastic, Vialon, and
Tecoflex, were evaluated in vitro for bacterial adherence after 2 and 24 hours' incubation
in trypticase soy broth and after 2 hours' incubation in nutrient-free phosphate buffer
(pH 7.2). The organisms used were Pseudomonas aeruginosa, Enterobacter aerogenes, Escherichia coli, and Staphylococcus epidermidis. The significant differences in in vitro adherence of the different bacterial species
to the various catheters were then evaluated in vivo by intravenous injection of a
single bolus of 1 × 105 organisms via tail vein of rats with previously placed catheters in their superior
venae cavae. There was no association between the in vitro bacterial adherence and
the tendency of the in vivo catheters to become colonized. Results of scanning electron
microscopy of clean catheters and those removed from the rats showed obvious differences
in surface characteristics and in clot adhesion between the catheters. These characteristics
did not correlate with bacterial adherence in vitro or colonization in vivo. It is
concluded that laboratory studies of bacterial adherence to, physical characteristics
of, and thrombogenicity of intravenous catheters do not necessarily translate into
resistance to clinical catheter sepsis.
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References
- Infections associated with intravascular lines.in: 6th ed. Current clinical topics in infectious diseases. Vol III. McGraw-Hill, 1982: 309-363
- Intravenous cannula associated infections.in: Bennett JV Brachman PS Hospital infections. Little, Brown & Company, Boston1979: 433-453
- Third day surgical fever.Arch Surg. 1971; 103: 158-166
- In vitro quantitative adherence of bacteria to intravascular catheters.J Surg Res. 1983; 34: 213-218
- A semiquantitative culture method for identifying intravenous-catheter-related infection.N Engl J Med. 1977; 296: 1305-1309
- Rapid diagnosis of intravascular catheter-associated infection by direct gram staining of catheter segments.N Engl J Med. 1985; 312: 1142-1147
- Statistical methods.in: 6th ed. Iowa State University Press, Ames, Iowa1967: 273-275
- Handbook of IV additive reviews. Hamilton Press, Hamilton, Ill1970: 18
- Mechanism of thrombogenesis during total parenteral nutrition: role of catheter composition.J Parenter Enteral Nutr. 1988; 12: 190-194
- Association between microorganism growth at the catheter insertion site and colonization of the catheter in patients receiving total parenteral nutrition.Surgery. 1982; 92: 720-727
- Catheter sepsis: the clue is the hub.Surgery. 1985; 97: 355-357
- Total parenteral nutrition-related infections.Am J Med. 1982; 73: 695-699
- Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters.J Infect Dis. 1982; 146: 479-482
- Pathogenesis and management of Staphylococcus epidermidis “plastic” foreign body infections.J Antimicrob Chem. 1984; 149 (D): 67-71
Article info
Publication history
Accepted:
October 17,
1988
Footnotes
☆Supported in part by a University of Michigan Biomedical Research Council grant.
Identification
Copyright
© 1989 Published by Elsevier Inc.
