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Abstract
Urogastrone (UG) administered subcutaneously increases the rate of intestinal regeneration
(neomucosal growth) on patched intestinal defects. Our purpose was to determine the
optimal route of delivery of UG for intestinal regeneration. In 22 New Zealand white
rabbits (2.1 to 3.4 kg) 2 × 5 cm ileal defects were patched with adjacent cecal serosal
surface. Group I (n = 6) served as controls. Group II (n = 5), group III (n = 6),
and group IV (n = 5) received UG, 1.5 μg/kg/hr, intravenously, subcutaneously, and
intraluminally via miniosmotic pumps. Neomucosal growth was assessed 7 days after
patching. Serum UG levels were detectable in only the intravenous group. Coverage
of the patched defect and neomucosal area was significantly greater and contraction
of the defect less in the groups receiving UG (p < 0.05). Neomucosal area was highest
in the intravenous group (286 ± 16 mm2), intermediate in groups III and IV (236 ± 19 and 215 ± 20 mm2), and lowest in the control group (152 ± 17 mm2; p < 0.05). Sucrase and maltase activities were significantly higher in the intravenous
group. Crypt cell production rate and ornithine decarboxylase activity were greater
in the UG-treated animals. UG stimulated intestinal regeneration by all routes of
delivery. The intravenous route had the greatest effect and was associated with the
highest serum levels of UG. These findings have implications for the mechanism of
the trophic effect of UG on the intestinal epithelium.
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Article info
Publication history
Accepted:
October 18,
1988
Footnotes
☆Supported by grant DK 36612 from the National Institutes of Health.
Identification
Copyright
© 1989 Published by Elsevier Inc.
