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Abstract
We studied the effect of a body burn on lung physiologic, biochemical, and histologic
changes in a 2-day postburn period. A 15% of total-body-surface third-degree burn
was produced in 24 adult sheep with lung and burn lymph fistulas. Eight sheep were
killed at 12 hours and eight at 48 hours. At 12 hours we noted increased lung tissue
lipid peroxidation, lung congestion, and neutrophil sequestration, in addition to
a 30% decrease in lung compliance. Lung permeability and water content were not increased.
Increased release of lipid peroxides and prostanoids were noted from burn tissue,
as evidenced by increased plasma levels of malondialdehyde and conjugated dienes that
remained elevated for about 8 hours and were decreased with wound removal. The lung
inflammatory response was still present at 48 hours, the cells being primarily neutrophils.
Nevertheless, the lipid peroxidation process, as measured by lung tissue malondialdehyde,
had resolved. There was no evidence of burn tissue infection, measured by quantitative
culture, to explain the persistent increase in lung inflammatory cells. Excision and
closure of the burn wound at 3 hours postburn in eight sheep attenuated the lipid
peroxidation and compliance changes but did not decrease the neutrophil sequestration.
We conclude that burn injury results in a local wound oxidant release that leads to
lipid peroxidation, both in wounds and in lung, as well as lung inflammation. The
lipid peroxidation process may be attenuated by removal of the wound. The neutrophil
sequestration is not altered, however, indicating this response occurs very early
after injury, probably as a result of oxidant-initiated complement activation.
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Article info
Publication history
Accepted:
October 21,
1988
Footnotes
☆Supported by National Institutes of Health grant GM31662.
Identification
Copyright
© 1989 Published by Elsevier Inc.
