This paper is only available as a PDF. To read, Please Download here.
Abstract
The effect of long-term cholesterol feeding on male rabbit gallbladder prostanoid
synthesis and bile phospholipid and bile acid levels was examined. In rabbits fed
a 2% cholesterol diet for 2, 5, 7, 9, 12, 15, 18, and 21 weeks, serum cholesterol
levels increased 12-fold or higher (68-fold at 21 weeks) when compared to the control
levels. Total prostanoid synthesis was significantly increased at 7 weeks or more
of cholesterol feeding with 6-keto PGF1α (PGI2 metabolite) and PGE2 as the major products. By 21 weeks of cholesterol feeding, 6-keto PGF1α and PGE2 levels were increased 15-fold and 11-fold higher, respectively, compared to the control.
Total bile phospholipid levels were significantly increased at 12 weeks or more of
cholesterol feeding, and total bile acids significantly increased only from 5 through
12 weeks of cholesterol feeding. These data show that long-term feeding male rabbits
with a 2% cholesterol diet increased endogenous gallbladder prostanoid synthesis,
which was associated with an increase in bile phospholipid levels. Increased rabbit
gallbladder bile phospholipids may represent a compensation of the animal for toxic
levels of tissue and bile cholesterol. Increased phospholipid levels in the bile or
tissue could serve as a source of increased availability of arachidonic acid leading
to a nonspecific increase in gallbladder prostanoid synthesis.
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to SurgeryAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Prostaglandins and the contractibility of the guinea pig biliary system.Pharmacology. 1981; 22: 24-30
- Triphasic effect of prostaglandins E1, E2, F2, and pentagastrin on the gallbladder pressure in dogs.Eur J of Pharmacol. 1975; 30: 107-112
- Inhibitory effect of prostaglandins on isosmotic fluid transport by rabbit gallbladder in vitro and its modification by blockade of endogenous PGE-biosynthesis with indomethacin.Acta Physiol Scand. 1974; 92: 456-507
- Prostacyclin (PGI2) biosynthesis and its effect on luminal fluid absorption in gallbladder.Surg Forum. 1984; 35: 229-230
- Effects of intra-arterial prostaglandin E2 on gallbladder fluid transport, motility and hepatic bile flow in the cat.Scand J Gastroenterol. 1981; 16: 1083-1088
- Arachidonic acid stimulates mucin secretion in prairie dog.Am J Physiol. 1983; 245: G92-G98
- Salicylate prevents gallbladder stasis and cholesterol gallstones in the prairie dog.Surg Forum. 1981; 32: 154-155
- Aspirin prevention of cholesterol gallstone formation in prairie dogs.Science. 1985; 211: 1429-1450
- Increases in gallbladder prostaglandin synthesis before the formation of cholesterol gallstones.Surgery. 1985; 98: 445-451
- Elevated prostaglandin production and mucus hypersecretion following rabbit bile duct ligation.Gastroenterology. 1987; 5 ([Abstract]): 1547
- The role of inflammation on male and female rabbit gallbladder biosynthesis.Gastroenterology. 1987; 5: 1548
- The role of inflammatory cells in enhanced rabbit gallbladder prostaglandin biosynthesis.Surg Forum. 1987; 3 ([Abstract]): 180-182
- Indomethacin inhibition of abnormal gallbladder in vitro fluid absorption in a rabbit model of early acute cholecystitis.Surg Forum. 1988; 39: 156-158
- Prostaglandin D2 formation by the gallbladder in experimental cholecystitis.Gut. 1986; 27: 370-373
- The role of prostaglandins in feline experimental cholecystitis.Surgery. 1985; 98: 760-767
- Exaggerated prostaglandin and thromboxane synthesis in the constricted renal vein of a rabbit.in: Samuelsson B Ramwell P Paoletti R Advances in prostaglandin and thromboxane research. Raven Press, New York1980: 1171
- Exaggerated prostaglandin and thromboxane synthesis in the renal vein constricted rabbit kidney modulators of renal resistance.Circ Res. 1980; 47: 231
- Effect of estrogen on rabbit gallbladder prostaglandin biosynthesis.J Surg Res. 1985; 38: 630-634
- Elevated PGI2 and PGE2 production in the rat ileum following mild hypotension.J Trauma. 1988; 28: 1202-1206
- Effects of sex hormones on rabbit gallbladder prostaglandin biosynthesis.J Surg Res. 1988; 45: 154-157
- Mechanism of enhanced renal prostaglandin biosynthesis in ureter obstruction: role of de novo protein synthesis.J Biol Chem. 1978; 253: 8120-8212
- Peptide-induced prostaglandin biosynthesis in the renal-vein-constricted kidney.Biochem J. 1981; 198: 357-363
- The arachidonic acid metabolic capacity of canine myocardium is increased during healing of acute myocardial infarction.Circ Res. 1982; 51: 743-750
- Further studies on the enzymatic conversion of prostaglandin endoperoxide into prostacyclin by porcine aorta microsomes.Biochem et Biophys Acta. 1978; 523: 250-262
- Regional localization of prostacyclin and thromboxane synthesis in dog stomach and intestinal tract.J Pharmacol Exp Ther. 1976; 211: 181-188
- Oxygenation of polyunsaturated fatty acids during prostaglandin biosynthesis by sheep vesicular glands.Biochemistry. 1972; 11: 3276-3285
- Evidence for two distinct forms of fatty acid cyclooxygenase in the brain.J Neurochem. 1982; 38: 1111-1117
- Protein measurement with the folin phenol reagent.J Biol Chem. 1951; 193: 265-275
- Application of imidazole as a selective inhibitor of thromboxane synthetase in human platelets.in: 2nd ed. Proc National Acad Sci USA. 74. 1977: 1716-1720
- Prostaglandins, oxygen tension and smooth muscle tone.Br J Pharmacol. 1972; 45: 451-462
- Prostaglandins in human seminal plasma.J Biol Chem. 1966; 241: 257-263
- Isolation and properties of intermediates in prostaglandin biosynthesis.Biochem Biophys Acta. 1973; 326: 448-461
- Ellefson RD Caraway WT Tietiz NW Fundamentals of clinical chemistry. 2nd ed. WB Saunders Co, Philadelphia1976: 506-509
- Glyco-7a,12a-dihydroxy-5B-cholanic acid as internal standard for high-pressure liquid chromatographic analysis of conjugated bile acids.J Lipid Res. 1985; 26: 1009-1011
- A simple method for the isolation and purification of total lipids from animal tissues.J Biol Chem. 1957; 226: 497-509
- Analysis of phospholipids by one-dimensional thin-layer chromatography.Liquid Chromatography. 1985; 3: 126-128
- Bile composition in the pregnant rabbit.Dig Dis. 1972; 17: 1-6
- The prostaglandin response to cholesterol feeding by the rabbit gallbladder in vitro.Surgery. 1988; 103: 69-73
- Phospholipids associated with vitamin C in experimental atherosclerosis.Arzneim-Forsch/Drug Research. 1980; 30: 627-630
- Homeostatic control of membrane cholesterol and fatty acid metabolism in the rat liver.Biochem J. 1988; 251: 11-16
- Influence of saturated and unsaturated fats on platelet fatty acids in cholesterol-fed rabbits.Atherosclerosis. 1987; 63: 85-96
- Effect of dietary cholesterol on phosphatidylcholines and phosphatidylethanolamines in bile and gallbladder mucosa in the prairie dog.Gastroenterology. 1989; 97: 1261-1267
- The composition of plasma lipoproteins and erythrocyte membranes in cholesterol-fed pigs with partial ileal bypass.Artery. 1987; 14: 165-189
Article info
Publication history
Accepted:
June 28,
1990
Footnotes
☆Supported by National Institutes of Health grant 38342.
Identification
Copyright
© 1991 Published by Elsevier Inc.
