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Abstract
Background. Intestinal fatty acid binding protein (I-FABP) is a 15 kd protein that constitutes
2% to 3% of enterocyte protein. Normally I-FABP is undetectable in serum. The purpose
of this study was to determine whether I-FABP could be detected in peripheral serum
and/or urine early in the evolution of intestinal ischemic injury.
Methods. I-FABP and two putative biochemical markers of mesenteric ischemia, hexosaminidase
and lactate dehydrogenase, were quantitated in the serum of rats subjected to mesenteric
ischemia produced by (1) 0.5 hours, 1 hour, or 3 hours of superior mesenteric artery
(SMA) occlusion followed by reperfusion; (2) 1 hour of mesenteric occlusion to a 10
cm segment of jejunum followed by reperfusion; and (3) arterial ligation to a 10 cm
segment of jejunum without reperfusion. I-FABP was also quantitated in the urine and
intestinal mucosa of these animals.
Results. The baseline serum I-FABP level was ⩽ 4.0 ng/ml in all animals. In control animals,
I-FABP remained unchanged throughout the experiment. In the ischemia/reperfusion groups,
I-FABP immediately appeared in the serum on reperfusion. With segmental arterial ligation,
I-FABP was detected in the serum within 15 minutes. Urinary content of I-FABP rose
60 minutes after its initial appearance in the serum, and its elimination paralled
serum I-FABP levels. Serum hexosaminidase and lactate dehydrogenase levels only rose
after 3 hours of SMA occlusion with reperfusion. One hour of SMA occlusion and reperfusion
resulted in only mild to moderate mucosal injury, whereas 3 hours of SMA ischemia
with reperfusion produced areas of transmural necrosis.
Conclusions. I-FABP is released into the peripheral circulation after reversible intestinal ischemic
injury and has potential as a biochemical marker to facilitate the early detection
of mesenteric ischemia.
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References
- Mesenteric ischemia.Surg Clin North Am. 1988; 68: 331-353
- The significance of elevation of serum phosphate levels in experimental intestinal ischemia.Surg Gynecol Obstet. 1981; 152: 593-596
- Acute intestinal infarction or obstruction: search for better laboratory tests in an animal model.Clin Chem. 1988; 34: 281-288
- Increased plasma malondialdehyde in patients with small intestinal strangulation obstruction.Acta Chir Scand. 1988; 154: 283-285
- Arterial levels of oxidized glutathione (GSSG) reflect oxidant stress in vivo.J Surg Res. 1990; 48: 291-296
- Effect of intestinal ischemia on diamine oxidase activity in rat intestinal tissue and blood.Gastroenterology. 1981; 80: 349-355
- Serum D(−)-lactate levels as an early predictor of acute intestinal ischemia in a rat model.in: Presented at the 25th Annual Meeting of the Association for Academic Surgery, Colorado Springs, Colo.November 20–23, 1991
- Experimental and clinical studies of mesenteric infarction.Surg Gynecol Obstet. 1981; 153: 360-362
- Hexosaminidase: a marker for intestinal gangrene in necrotizing enterocolitis.J Pediatr Surg. 1983; 18: 449-452
- Nuclear magnetic resonance as a noninvasive method of diagnosing intestinal ischemia: technique and preliminary results.J Pediatr Surg. 1991; 26: 775-779
- The use of infrared photoplethysmography in identifying early intestinal ischemia.Arch Surg. 1987; 122: 308-310
- The use of intraperitoneal xenon for early diagnosis of acute mesenteric ischemia.Surgery. 1983; 95: 404-411
- Circulating concentrations of porcine ileal peptide but not hexosaminidase are elevated following 1 hour of mesenteric ischemia.J Surg Res. 1988; 45: 134-139
- Fatty acid binding protein in small intestine: identification, isolation, and evidence for its role in cellular fatty acid transport.J Clin Invest. 1974; 54: 326-338
- The recovery of function and microcirculation in small intestinal loops following ischaemia.Gut. 1972; 13: 784-789
- The human and rodent intestinal fatty acid binding protein genes: a comparative analysis of their structure, expression, and linkage relationships.J Biol Chem. 1987; 262: 16060-16071
- Tissue expression of three structurally different fatty acid binding proteins from rat heart muscle, liver, and intestine.Biochem Biophys Res Commun. 1986; 137: 929-935
- Early detection of small intestinal ischemia by elevated circulating intestinal fatty acid binding protein (I-FABP).in: 1st ed. Surg Forum. 42. 1991: 118-120
- Elevation of circulating intestinal fatty acid binding protein in a luminal contents-initiated model of NEC.J Pediatr Surg. 1993; 28: 1-5
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal Biochem. 1976; 72: 248-254
- Tay-Sachs disease: detection of heterozygotes and homozygotes by serum hexosaminidase assay.N Engl J Med. 1979; 152: 593-597
- Neonatal necrotizing enterocolitis in the absence of pneumatosis intestinalis.Am J Dis Child. 1982; 136: 618-620
- Changing trends in necrotizing enterocolitis: experience with 302 cases in two decades.Ann Surg. 1991; 214: 300-307
- Advances in the assessment of disease activity in inflammatory bowel disease.in: 1st ed. Mayo Clin Proc. 64. 1989: 800-807
- A possible serum marker for rejection after small intestine transplantation.Am J Surg. 1987; 153: 68-74
- Acute mesenteric ischemia.Surgery. 1982; 92: 646-653
- Analyzing the structures, functions and evolution of two abundant gastrointestinal fatty acid binding proteins with recombinant DNA and computational techniques.Chem Phys Lipids. 1985; 38: 137-158
- Translational control of the circadian rhythm of liver sterol carrier protein: analysis of mRNA sequences with a specific cDNA probe.J Biol Chem. 1985; 260: 5435-5439
Article info
Publication history
Accepted:
February 7,
1992
Identification
Copyright
© 1993 Published by Elsevier Inc.
