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Original communication| Volume 113, ISSUE 5, P564-573, May 1993

Epidermal growth factor limits structural alterations in gastrointestinal tissues and decreases bacterial translocation in burned mice

  • Ramon L. Zapata-Sirvent
    Affiliations
    From the Departments of Surgery and Pathology, University of California-San Diego Medical Center, San Diego, Calif., USA

    From the Amgen, Inc., Thousand Oaks, Calif., USA
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  • John F. Hansbrough
    Correspondence
    Reprint requests: John F. Hansbrough, MD, Department of Surgery H640B, U.C.S.D. Medical Center, 225 Dickinson St., San Diego, CA 92103.
    Affiliations
    From the Departments of Surgery and Pathology, University of California-San Diego Medical Center, San Diego, Calif., USA

    From the Amgen, Inc., Thousand Oaks, Calif., USA
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  • Paul Wolf
    Affiliations
    From the Departments of Surgery and Pathology, University of California-San Diego Medical Center, San Diego, Calif., USA

    From the Amgen, Inc., Thousand Oaks, Calif., USA
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  • Leila S. Grayson
    Affiliations
    From the Departments of Surgery and Pathology, University of California-San Diego Medical Center, San Diego, Calif., USA

    From the Amgen, Inc., Thousand Oaks, Calif., USA
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  • Margery Nicolson
    Affiliations
    From the Departments of Surgery and Pathology, University of California-San Diego Medical Center, San Diego, Calif., USA

    From the Amgen, Inc., Thousand Oaks, Calif., USA
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DOI:https://doi.org/10.5555/uri:pii:0039606093901203
Epidermal growth factor limits structural alterations in gastrointestinal tissues and decreases bacterial translocation in burned mice
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      Abstract

      Background. Burn injury produces acute gastrointestinal derangements that may predispose to bacterial translocation (BT). We studied effects of recombinant human epidermal growth factor (r-HuEGF), a gastrointestinal trophic hormone, on gastrointestinal alterations and BT after murine burn injury.
      Methods. r-HuEGF was administered 1 and 12 hours after burn injury in a dose of 4 μg per animal subcutaneously after 25% and 32% total body surface area (TBSA) scald burn. Small bowel and gastric weight and histologic factors were studied, and BT was measured by culturing mesenteric lymph nodes.
      Results. Mice treated with r-HuEGF maintained gastric and small intestine weight, measured 24 hours after burn injury, and ileal mucosal height was preserved, whereas burned-untreated mice lost organ weight and mucosal height. BT was decreased significantly in mice with 32% TBSA burn injury treated with r-HuEGF after injury (burn, 64.2% of animals had BT; burn-r-HuEGF, 34.6% had BT; p < 0.05). After 25% TBSA burn injury, BT was also decreased in r-HuEGF-treated animals (burn, 31.4% of animals had BT; burn-r-HuEGF, 14.3% had BT), but the difference was not statistically significant (p < 0.1).
      Conclusions. r-HuEGF improves intestinal and gastric structure in mice 24 hours after burn injury and decreases BT after 32% TBSA burn injury.
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      Article info

      Publication history

      Accepted: February 1, 1992

      Footnotes

      ☆Supported in part by National Institutes of Health grant GM 08247.

      Identification

      DOI: https://doi.org/10.5555/uri:pii:0039606093901203

      Copyright

      © 1993 Published by Elsevier Inc.

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