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Abstract
Background. Lymphocytes play an important role in the activation of polymorphonuclear neutrophil
(PMN) antimicrobial functions. This study was undertaken to evaluate the ability of
lymphocytes from severely injured adults with elevated Candida antigen titers to activate
the anticandidal function of normal PMNs.
Methods. Lymphocytes from 13 injured adults with elevated Candida antigen titers (titer+)
were isolated and incubated with or without heat-killed C. albicans. After a 48-hour
incubation, cell culture supernatants were harvested and tested for the ability to
activate the anticandidal function of normal PMNs. Lymphocytes from 13 volunteers
and 13 injury-matched patients with negative Candida antigen titers (titer—) were
studied for comparison.
Results. The patients with titer+ and titer— were well matched in terms of age, sex, Injury
Severity Score, units of blood transfused, and length-breadth of antibiotic therapy.
Patients with titer+ had a higher incidence of bacterial infections than did patients
with titer-. Only 2 of the 13 patients with titer+ had a positive Candida culture.
Lymphocytes from the volunteers and patients with titer- released large amounts of
a PMN-activating factor (s) when exposed to C. albicans. Lymphocytes from the patients
with titer+ were defective in their ability to release this activating factor(s) after
exposure to C. albicans.
Conclusions. Although culture evidence of Candida was uncommon, injured adults with elevated Candida
antigen titers have lymphocyte dysfunction and often suffer documented bacterial infections.
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References
- Antigens and immune response in Candida albicans infection.Immunol Cell Biol. 1990; 68: 1-13
- High incidence of fungus infections in renal transplantation patients treated with antilymphocyte and conventional immunosuppression.Transplant Proc. 1973; 5: 549-553
- Opportunistic infections and immune deficiency in homosexual men.Ann Intern Med. 1982; 96: 700-704
- Opportunistic fungal infection among cancer patients: a ten year autopsy study.Am J Clin Pathol. 1979; 72: 617-621
- Damage to Candida albicans hyphae and pseudohyphae by the myeloperoxidase system and oxidative products of neutrophil metabolism in vitro.J Clin Invest. 1980; 66: 908-917
- Regulation of human polymorphonuclear neutrophil activity against Candida albicans by large granular lymphocytes via release of a PMN-activating factor.J Immunol. 1987; 139: 2761-2767
- Production of granulocyte-macrophage colony stimulating factor by large granular lymphocytes stimulated by Candida albicans: role in activation of human neutrophil function. 1991; 77: 2259-2265
- Candida sepsis: pathogenesis and principles of treatment.Ann Surg. 1974; 179: 697-711
- Indications for therapy for fungemia in postoperative patients.Arch Surg. 1982; 117: 1272-1275
- Evaluation of a Candida antigen detection test (Cand-TecTM) in the diagnosis of deep candidiasis in neutropenic patients.Eur J Cancer Clin Oncol. 1988; 24: 1655-1659
- Prospective evaluation of a Candida antigen detection test for invasive candidiasis in immunocompromised adult patients with cancer.Am J Med. 1989; 87: 621-627
- Value of the Cand-Tec Candida antigen assay in the diagnosis and therapy of systemic candidiasis in high-risk patients.Eur J Clin Microbiol Infect Dis. 1989; 8: 770-777
- Comparison of antibody, antigen, and metabolic assays for hospitalized patients with disseminated or peripheral candidiasis.J Clin Microbiol. 1990; 28: 905-909
- Collaborative evaluation of antigen detection by a commercial latex agglutination test and enzyme immunoassay in the diagnosis of invasive candidiasis.J Clin Microbiol. 1990; 28: 249-253
- Retrospective evaluation of two latex agglutination tests for detection of circulating antigens during invasive candidosis.J Clin Microbiol. 1992; 30: 2158-2164
- Implications of Candida antigen titers in injured adults.Contemp Surg. 1993; 42: 327-332
- Impaired polymorphonuclear leukocyte anticandidal function in injured adults with elevated Candida antigen titers.Arch Surg. 1993; 128: 40-46
- A rapid [3H] glucose incorporation assay for determination of lymphoid cell-mediated inhibition of Candida albicans growth.J immunol Methods. 1986; 92: 73-77
- Different effects of native Candida albicans mannan and mannan-derived oligosaccharides on antigen stimulated lymphoproliferation in vitro.J Immunol. 1990; 144: 707-716
- Pathogenesis of candidiasis: immunosuppression by cell wall mannan catabolites.Arch Surg. 1989; 124: 1290-1294
- Chronic mucocutaneous candidiasis.Ann Rev Med. 1981; 32: 491-493
- Serodiagnosis of candidiasis, aspergillosis, and cryptococcosis.Clin Infect Dis. 1992; 14 (suppl): S11-S22
Article info
Footnotes
☆Supported in part by a grant from the James N. Patterson Research and Education Fund.
☆☆Presented at the Fiftieth Annual Meeting of the Central Surgical Association, Cincinnati, Ohio, March 4–6, 1993.
Identification
Copyright
© 1993 Published by Elsevier Inc.
