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Abstract
Background. Ras oncogene mutations have been found in many human cancers; however, pancreatic
endocrine tumors have rarely been studied. The purpose of this study was to analyze
ras mutations in pancreatic endocrine tumors and to compare these results with the
incidence of ras mutations in pancreatic exocrine cancers studied in our laboratory.
Methods. Ras oncogene mutations were studied in 33 foregut endocrine tumors (pancreatic 31,
duodenal submucosa 2). Eleven were insulinomas, 12 gastrinomas, 2 glucagonomas, and
11 others were nonfunctioning islet cell carcinomas. Thirteen were benign and 20 were
malignant. These were compared with 65 pancreatic exocrine cancers. Tumors were microdissected
from paraffin-embedded sections. DNA was extracted and amplified by polymerase chain
reaction. Mutations were detected by a oligonucleotide hybridization method with sequence-specific
phosphorus 32-radiolabeled probes.
Results. No ras mutations were identified among the 33 pancreatic endocrine tumors. In contrast,
51 of 65 (78.5%) pancreatic exocrine cancers exhibited a ras mutation. Fifty were
K-ras mutations and one unusual tumor exhibited a N-61 ras mutation.
Conclusions. Ras oncogene mutations do not play a role in the tumorigenesis of pancreatic endocrine
tumors.
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References
- Ras oncogenes in human cancer: a review.Cancer Res. 1989; 49: 4682-4689
- Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes.Cell. 1988; 53: 549-554
- KRAS codon 12 mutations occur very frequently in pancreatic adenocarcinomas.Nucleic Acids Res. 1988; 16: 7773-7782
- High frequency of Kiras codon 12 mutations in pancreatic adenocarcinomas.Int J Cancer. 1989; 43: 1037-1041
- Frequent glycine-to-aspartic acid mutations at codon 12 of c-Ki-ras gene in human pancreatic cancer in Japan.Jpn J Cancer Res. 1990; 81: 135-140
- Analysis of ras gene mutations in biliary and pancreatic tumors by polymerase chain reaction and direct sequencing.Cancer. 1990; 66: 930-935
- Detection of c-K-ras mutations in fine needle aspirates from human pancreatic adenocarcinomas.Cancer Res. 1990; 50: 1279-1283
- Distinguishing pancreatic carcinoma from other periampullary carcinomas by analysis of mutations in the Kirstenras oncogene.Ann Surg. 1991; 214: 657-662
- Mutations in the K-ras2 oncogene during progressive stages of human colon carcinoma.in: Proc Natl Acad Sci USA. 86. 1989: 2403-2407
- Prevalence of ras mutations in human colorectal cancers.Nature. 1987; 327: 293-297
- Incidence and possible clinical significance of K-ras oncogene activation in adenocarcinoma of the lung.Cancer Res. 1988; 48: 5738-5741
- Ras mutations in endocrine tumors: mutation detection by polymerase chain reaction-single strand conformation polymorphism.Jpn J Cancer Res. 1992; 83: 1057-1062
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques.J Histochem Cytochem. 1981; 29: 577-580
- Purification of DNA from formaldehyde-fixed and paraffin-embedded human tissue.Biochem Biophys Res Commun. 1985; 130: 118-126
- N-ras 61 oncogene mutations in Hurthle cell tumors.Surgery. 1990; 108: 994-1000
- High frequency of ras oncogene activation in all stages of human thyroid tumorigenesis.Oncogene. 1989; 4: 159-164
- The value of measurement of ras oncogenes and nuclear DNA analysis in the diagnosis of Hurthle cell tumors of the thyroid.World J Surg. 1992; 16: 745-752
- Allele loss on chromosome 10 and point mutations of ras oncogenes are infrequent in tumors of MEN 2A.Henry Ford Hosp Med J. 1989; 32: 112-115
- Low frequency of ras gene mutations in neuroblastomas, pheochromocytomas, and medullary thyroid cancers.Cancer Res. 1991; 51: 1596-1599
- Ras mutations in human pituitary tumors.J Clin Endocrinol Metab. 1992; 74: 914-919
- Genetic abnormalities in sporadic parathyroid adenomas.J Clin Endocrinol Metab. 1990; 71: 293-297
- Activation of the c-K-ras oncogene in a human pancreas carcinoma.Biochem Biophys Res Commun. 1985; 127: 168-174
Article info
Footnotes
☆Supported in part by a grant from the Nathan and Frances Goldblatt Society for Cancer Research.
☆☆Presented at the Fiftieth Annual Meeting of the Central Surgical Association, Cincinnati, Ohio, March 4–6, 1993.
Identification
Copyright
© 1993 Published by Elsevier Inc.
