Original communication| Volume 113, ISSUE 6, P624-630, June 1993

Activation state of polymorphonuclear leukocytes in surgical patients: Characterization of surface receptor expression

  • Jose M. Tellado
    Departments of Surgery, Immunology, and Microbiology, McGill University, Montreal, Quebec, Canada
    Search for articles by this author
  • Nicolas V. Christou
    Reprint requests: Nicholas V. Christou, MD, Department of Surgery, Royal Victoria Hospital, 687 Pine Ave. W., Montreal, Quebec, Canada H3A 1A1.
    Departments of Surgery, Immunology, and Microbiology, McGill University, Montreal, Quebec, Canada
    Search for articles by this author
  • Author Footnotes
    a Present address: Cirugia General III, Hospital “Gregorio Maranon,” c/Dr. Esquerdo 46, 28007 Madrid, Spain.
    a1 Recipient of the Lederle Fellowship on Surgical Infectious Diseases from the Canadian Surgical Research Fund.
      This paper is only available as a PDF. To read, Please Download here.


      Background. We studied the expression of chemotactic, opsonic, and adherent receptors on the membranes of polymorphonuclear neutrophils (PMNs) from surgical patients because modulation of these receptors has been suggested in the control of critical PMN functions that directly or indirectly influence patient outcome.
      Methods. Healthy subjects who had PMN adherence, chemotaxis, and receptor measurements were compared with preoperative surgical patients and those who were within 48 hours of an admission to an intensive care unit (surgical intensive care unit) because of an acute illness.
      Results. The following receptor/cell pattern was found in control subjects, preoperative patients, and patients in the surgical intensive care unit, respectively: formyl peptide (13,000 vs 18,000 vs 22,000), CR3 (59,000 vs 105,000 vs 121,000), fibronectin (21,000 vs 20,000 vs 35,000), FcIIγ (9,000 vs 20,000 vs 25,000), C5a (347 vs 265 vs 250 mean channel number), and FcIIIR (64,000 vs 75,000 vs 26,000). This receptor pattern was partly correlated directly or indirectly with the acute-phase response, neutrophil adherence, and PMN chemotaxis from these subjects.
      Conclusions. The data suggest that there are alterations in the expression and modulation of PMN surface membrane receptors in patients with a “stable” disease process compared with those with an acute illness, which may affect critical PMN functions needed to combat bacterial infections.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Surgery
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Christou NV
        • Tellado JM
        In vitro polymorphonuclear neutrophil function in surgical patients does not correlate with anergy but with “activating”: processes such as sepsis or trauma.
        Surgery. 1989; 106: 718-722
        • Tellado JM
        • Christou NV
        Critically ill anergic patients demonstrate polymorphonuclear neutrophil activation in the intravascular compartment with decreased cell delivery to inflammatory focci.
        J Leukoc Biol. 1991; 50: 547-553
        • Ward PA
        • Warren JS
        • Johnson KJ
        Leukocytic oxygen products and their diverse biological effects.
        in: Movat HZ Leukocyte migration and its sequelae. Karger, Basel1987: 161-168
        • Knauss WA
        • Draper EA
        • Wagner DP
        • Zimmerman JE
        A severity of disease classification system.
        Crit Care Med. 1983; 13: 818-829
        • Fay SP
        • Posner RG
        • Swann WN
        • Sklar LA
        Real-time analysis of the assembly of ligand, receptor, and G protein by quantitative fluorescence flow cytometry.
        Biochemistry. 1991; 30: 5066-5075
        • Schwartz JR
        Fluorescent microbead standards.
        Flow Cytometry Standards, Research Triangle Park, North Carolina1988
        • Christou NV
        • Meakins JL
        Neutrophil function in anergic surgical patients: neutrophil adherence and chemotaxis.
        Ann Surg. 1979; 190: 557-564
        • Falk W
        • Goodwin RH
        • Leonard AJ
        A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration.
        J Immunol Methods. 1980; 20: 833-841
        • Singer IL
        • Scott L
        • Kawka DW
        • Kazazis DM
        Adhesomas: specific granules containing receptors for laminin, C3bi/fibrinogen, fibronectin and vitronectin in human polymorphonuclear leukocytes and monocytes.
        J Cell Biol. 1989; 109: 3169-3182
        • Snyderman R
        • Smith CD
        • Verghese MW
        Model for leukocyte regulation by chemoattractant receptors: roles of a guanine nucleotide regulatory protein and polyphosphoinositide metabolism.
        J Leukoc Biol. 1986; 40: 785-800
        • Nelson RD
        • Hasslen SR
        • Ahrenholz DH
        • Solem LD
        Influence of minor thermal injury on expression of complement receptor CR3 on human neutrophils.
        J Burn Care Rehabil. 1987; 8: 496-502
        • Buyon JP
        • Shadick N
        • Berkman R
        • et al.
        Surface expression of Math Eq, the complement receptor type 3, CR3, as a marker of disease activity in systemic lupus erythematosus.
        Clin Immunol Immunopathol. 1988; 46: 141-147
        • Vedder NB
        • Harlan JM
        Increased surface expression of Math Eq (MAC-1) is not required for stimulated neutrophil adherence to cultured endothelium.
        J Clin Invest. 1988; 81: 676-682
        • Smith CW
        • Marlin SD
        • Rothlein R
        • Tomam C
        • Anderson DC
        Comparative interactions of LFA-1 and MAC-1 with intercellular adhesion molecule-1 in facilitating adherence and transendothelial migration of human neutrophils in vitro.
        J Clin Invest. 1989; 83: 2008-2017
        • Nuytinck HK
        • Offermans XJ
        • Kubat K
        • Goris JA
        Whole-body inflammation in trauma patients: an autopsy study.
        Arch Surg. 1988; 123: 1519-1524
        • Brown EJ
        • Goodwin JL
        Fibronectin receptors of phagocytes: characterization of the Arg-Gly-Asp binding proteins of human monocytes and polymorphonuclear leukocytes.
        J Exp Med. 1988; 167: 777-793
        • Miura S
        • Shirakami A
        • Ohara A
        • Ohara T
        • Saito S
        Fibronectin receptor on polymorphonuclear leukocytes in families of Ehlers-Danlos syndrome and other hereditary connective tissue disease.
        J Lab Clin Med. 1990; 116: 363-368
        • Van Epps DE
        • Simpson S
        • Bender JG
        • Chenoweth DE
        Regulation of C5a and formyl peptide receptor expression on human polymorphonuclear leukocytes.
        J Immunol. 1990; 144: 1062-1068
        • Heideman M
        • Hugli TE
        Anaphylatoxin generation in multisystem organ failure.
        J Trauma. 1984; 24: 1038-1043
        • Solomkin JS
        • Cotta LA
        • Ogle JD
        • et al.
        Complement-induced expression of cryptic receptors on the neutrophil surface: a mechanism for regulation of acute inflammation in trauma.
        Surgery. 1984; 96: 336-342
        • Fletcher MP
        • Gallin JI
        Human neutrophils contain an intracellular pool of putative receptors for the chemoattradtant N-formyl-methionyl-leucyl-phenylalanine.
        Blood. 1983; 62: 792-799
        • Huizinga TWJ
        • Roos D
        • von dem Borne EG
        Neutrophil Fc-Y receptors: a two way bridge in the immune system.
        Blood. 1990; 75: 1211-1214
        • Petty HR
        • Francis JW
        • Anderson CL
        Cell surface distribution of Fc receptors II and III on living human neutrophils before and during antibody dependent cellular cytotoxicity.
        J Cell Physiol. 1989; 141: 598-605
        • Huizinga TW
        • van Kemenada F
        • Koenderman L
        • et al.
        The 40-kDa Fc gamma receptor (FcRII) on human neutrophils is essential for the IgG-induced respiratory burst and IgG-induced phagocytosis.
        J Immunol. 1989; 142: 2365-2369
        • Huizinga TW
        • van der Schoot CE
        • Jost CK
        • et al.
        The PI-linked receptor FCrIII is released on stimulation of neutrophils.
        Nature. 1989; 333: 565-567