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Abstract
Background. Somatostatin analogues inhibit peptide release and cell growth through multiple postreceptor
signal transduction mechanisms (PRSTM), including G proteins (GP), cyclic adenosine
monophosphate (cAMP), calcium, protein kinase C (PKC), and tyrosine phosphatase (TP).
Octreotide acetate (OA), a somatostatin analogue, has been shown to inhibit angiogenesis;
however, the PRSTM involved are unknown.
Methods. Fertilized chicken eggs were obtained and incubated. On day 3, embryos were removed
and placed in plastic wrap hammocks. On day 7, disks containing OA, test substances
that interfere with PRSTM, or combinations of OA plus a. test substance were placed
on the developing chorioallantoic membrane. Blood vessel growth under each disk was
assessed at 24 hours. Data were evaluated by chi-squared analysis.
Results. OA's ability to inhibit angiogenesis is significantly diminished when combined with
calcium, bradykinin (increases calcium), pertussis toxin (inhibits GP), or 3-isobutyl-1
methylxanthine (increases cAMP). In contrast, no significant decrease is noted in
OA's ability to inhibit angiogenesis when combined with phorbol ester (activates PKC)
or vanadale (inhibits TP).
Conclusions. OA-induced inhibition of angiogenesis is GP, calcium, and cAMP dependent and is PKC
and TP independent. Better understanding of the PRSTM involved with OA-induced inhibition
of angiogenesis may lead to enhancement of OA's effect on angiogenesis.
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Article info
Footnotes
☆Presented at the Fifteenth Annual Meeting of the American Association of Endocrine Surgeons, Dearborn, Mich., April 17–19, 1994.
Identification
Copyright
© 1994 Published by Elsevier Inc.
