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Research Article| Volume 116, ISSUE 6, P1153-1158, December 1994

Peptide YY immunoneutralization inhibits meal-induced absorption in vivo

  • Anton J. Bilchik
    Affiliations
    From the Department of Surgery, University of California at Los Angeles and Sepulveda Veterans Affairs Medical Centers, and Center for Ulcer Research and Education, Los Angeles, Calif., USA

    Department of Physiology, Creighton University, Omaha, Neb., USA
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  • Oscar J. Hines
    Affiliations
    From the Department of Surgery, University of California at Los Angeles and Sepulveda Veterans Affairs Medical Centers, and Center for Ulcer Research and Education, Los Angeles, Calif., USA

    Department of Physiology, Creighton University, Omaha, Neb., USA
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  • Stanley W. Ashley
    Affiliations
    From the Department of Surgery, University of California at Los Angeles and Sepulveda Veterans Affairs Medical Centers, and Center for Ulcer Research and Education, Los Angeles, Calif., USA

    Department of Physiology, Creighton University, Omaha, Neb., USA
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  • Thomas E. Adrian
    Affiliations
    From the Department of Surgery, University of California at Los Angeles and Sepulveda Veterans Affairs Medical Centers, and Center for Ulcer Research and Education, Los Angeles, Calif., USA

    Department of Physiology, Creighton University, Omaha, Neb., USA
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  • John Walsh
    Affiliations
    From the Department of Surgery, University of California at Los Angeles and Sepulveda Veterans Affairs Medical Centers, and Center for Ulcer Research and Education, Los Angeles, Calif., USA

    Department of Physiology, Creighton University, Omaha, Neb., USA
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  • Helen Wong
    Affiliations
    From the Department of Surgery, University of California at Los Angeles and Sepulveda Veterans Affairs Medical Centers, and Center for Ulcer Research and Education, Los Angeles, Calif., USA

    Department of Physiology, Creighton University, Omaha, Neb., USA
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  • Carson D. Liu
    Affiliations
    From the Department of Surgery, University of California at Los Angeles and Sepulveda Veterans Affairs Medical Centers, and Center for Ulcer Research and Education, Los Angeles, Calif., USA

    Department of Physiology, Creighton University, Omaha, Neb., USA
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  • Michael J. Zinner
    Affiliations
    From the Department of Surgery, University of California at Los Angeles and Sepulveda Veterans Affairs Medical Centers, and Center for Ulcer Research and Education, Los Angeles, Calif., USA

    Department of Physiology, Creighton University, Omaha, Neb., USA
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  • David W. McFadden
    Correspondence
    Reprint requests: David W. McFadden, MD, Associate Professor of Surgery, Department of Surgery, Division of General Surgery, 10833 Le Conte Ave. (72-215 CHS), Los Angeles, CA 90024.
    Affiliations
    From the Department of Surgery, University of California at Los Angeles and Sepulveda Veterans Affairs Medical Centers, and Center for Ulcer Research and Education, Los Angeles, Calif., USA

    Department of Physiology, Creighton University, Omaha, Neb., USA
    Search for articles by this author
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      Abstract

      Background. Plasma peptide YY (PYY) levels rise after a meal and have recently been shown to increase small bowel-absorption. The purpose of this study was to determine whether immunoneutralization of PYY would block postprandial absorption in vivo.
      Methods. Exteriorized, neurovascularly intact jejunal and ileal segments (25 cm) were created in six mongrel dogs. After a 2-week recovery luminal perfusion with an isotonic buffer, containing [14C] polyethylene glycol as a volume marker, was used to analyze water and sodium flux after an oral meal. Each meal was accompanied by either intravenous anti-PYY (0.5 mg · kg−1 · h−1) or nonspecific immunoglobulin IG (control). PYY antibody binding was determined by radioimmunoassay.
      Results. Displacement studies showed complete PYY neutralization. In control experiments feeding increased absorption of sodium and water in both segments. PYY immunoneutralization had no effect on jejunal absorption but significantly diminished ileal absorption (p < 0.05).
      Conclusions. These results suggest that PYY acts selectively in the ileum to increase postprandial fluid and electrolyte absorption after a meal. Agents directed at PYY-stimulated absorption may prove to be of therapeutic benefit in patients with malabsorptive conditions.
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