Abstract
Background. Endocrine allografts are an option for the treatment of endocrine failure. Methods. One lobe of the thyroid was transplanted under the kidney capsule. Results. C57BL/10 (H2b) thyroids were rejected in naive CBA (H2k) mice within 14 days after transplantation. When mice were treated with anti-CD4
monoclonal antibodies (mAb), all grafts survived for more than 60 days. The first
grafts still survived after second C57BL/10 or Balb/c (H2d) thyroid grafts that were transplanted into the same recipients were rejected acutely,
which suggests that the primary grafts were modified under anti-CD4 mAb treatment.
To confirm this hypothesis, C57BL/10 thyroid grafts from anti-CD4 mAb-treated mice
were retransplanted. All grafts survived in naive mice; this correlated with the overexpression
of heme oxygenase-1 (HO-1) in the grafts. Next, an inhibitor of HO-1 (zinc protoporphyrin)
or control compound (copper protoporphyrin) was injected intraperitoneally after transplantation
of C57BL/10 thyroid grafts into the primary CBA recipients that had been treated with
anti-CD4 mAb. The grafts in mice that had been treated with zinc protoporphyrin, but
not copper protoporphyrin, were rejected when retransplanted to naive recipients.
Conclusions. Overexpression of HO-1 correlated with the protection of fully allogeneic thyroid
grafts from rejection when retransplanted into naive recipients. (Surgery 2000;128:910-7.)
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Article info
Footnotes
*Reprint requests: Masanori Niimi, MD, PhD, First Department of Surgery, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
**Surgery 2000;128:910-7.
Identification
Copyright
© 2000 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
