Abstract
Background: Hepatocyte transplantation is an attractive potential treatment for liver-based inborn
errors of metabolism and for fulminant hepatic failure. Dalmatian dogs have a metabolic
error that results in hyperuricosuria. This report focuses on the effect of multiple,
sequential intrasplenic transplants of fresh and cryopreserved hepatocytes in dalmatians.
Methods: Dalmatians underwent intrasplenic hepatocyte transplantation with hepatocytes taken
from healthy mongrels. Dalmatian urinary uric acid excretion was measured preoperatively,
and this served as the control value. Three hepatocyte transplantations were performed
at 30-day intervals—the first with freshly isolated cells,and both the second and
the third with cryopreserved hepatocytes from the same donor. Urinary uric acid excretion
was measured postoperatively twice per week. Results: The urinary uric acid excretion decreased an average of 54% after the first hepatocyte
transplantation. The effect was transient and lasted an average of 22 days (range,
19-50 days). Subsequent intrasplenic hepatocyte transplantation with cryopreserved
hepatocytes resulted in similar decreases in urinary uric acid excretion. Each transplant
resulted in a significant decrease in urinary uric acid excretion when compared with
baseline values (P = <.001). Conclusions: Sequential intrasplenic hepatocyte transplantation is feasible in this model. This
method provided a significant, but transient, correction in urinary uric acid excretion
that was similar with either fresh or cryopreserved hepatocytes. A substantial biologic
effect provided by cryopreserved hepatocytes has important implications in clinical
hepatocyte transplantation. (Surgery 2000;127:193-9.)
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Article info
Publication history
Accepted:
July 28,
1999
Footnotes
*Supported in part by the Eleanor B. Pillsbury Foundation.
**Reprint requests: Enrico Benedetti, MD, Division of Transplantation, University of Illinois at Chicago, 840 S Wood St M/C 958, Chicago, IL 60612.
Identification
Copyright
© 2000 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
