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1Diverse diagnostic procedures for the detection of micrometastasis at the genetic level have developed rapidly. RT-PCR is one of the most sensitive methods to detect a small number of circulating tumor cells in blood and can detect a single tumor cell among 105 to 107 normal cells.
- Hayashi N
- Egami H
- Kai M
- Kurusu Y
- Takano S
- Ogawa M
No-touch isolation technique reduces intraoperative shedding of tumor cells into the portal vein during resection of colorectal cancer.
Surgery. 1999; 125: 369-374
2Despite the immense power of this technique, the occurrence of false-positive results is a big problem. Concerning the DNA-based method, amplification of tumor-specific molecular markers by PCR is also able to offer a sensitive way to detect low copy numbers of such mutations. The mutant allele-specific amplification (MASA) method is one technique of PCR amplification of tumor-specific DNA sequences that can detect 1 tumor cell with genetic alteration among 104 normal cells.
- Smith B
- Selby P
- Southgate J
- Pittman K
- Bradley C
- Blair GE
Detection of melanoma cells in peripheral blood by means of reverse transcriptase and polymerase chain reaction.
Lancet. 1991; 338: 1227-1229
- Takeda S
- Ichii S
- Nakamura Y
Detection of K-ras mutation in sputum by mutant-allele-specific amplification (MASA).
Hum Mutat. 1993; 2: 112-117
4Despite the limitation of sensitivity compared with other methods, we have used MASA to detect circulating tumor cells selectively in the portal blood stream to avoid false-positive results.
- Hayashi N
- Arakawa H
- Nagase H
- Yanagisawa A
- Kato Y
- Ohta H
- et al.
Cancer Res. 1994; 54: 3853-3856
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- No-touch isolation technique reduces intraoperative shedding of tumor cells into the portal vein during resection of colorectal cancer.Surgery. 1999; 125: 369-374
- Detection of melanoma cells in peripheral blood by means of reverse transcriptase and polymerase chain reaction.Lancet. 1991; 338: 1227-1229
- Detection of K-ras mutation in sputum by mutant-allele-specific amplification (MASA).Hum Mutat. 1993; 2: 112-117
- Cancer Res. 1994; 54: 3853-3856
- Blood dissemination of colonic epithelial cells during no-touch surgery for rectosigmoid cancer.Lancet. 1999; 354: 392
© 2000 Mosby, Inc. Published by Elsevier Inc. All rights reserved.