Abstract
Background. Previous work has demonstrated that intestinal ischemia increases plasma amylin concentration.
This study examined the relationship between the degree of intestinal ischemia injury
and plasma amylin in an experimental rat model. Methods. Wistar rats were divided into a control group (n = 6); a sham-operated group (n =
9); and 3 intestinal ischemia-reperfusion groups (n = 8 in each), which underwent
clamping of the superior mesenteric artery for either 15, 30, or 45 minutes followed
by 15 minutes of reperfusion. Samples were then collected for intestinal histology
and measurement of amylin, insulin, and glucose. Results. There was a positive correlation between the histologic score of the intestinal injury
and the measured plasma amylin concentration (R = 0.48, P =.007). The median plasma concentration of amylin was 62 pmol/L (range, 42-97 pmol/L)
in the 30-minute intestinal ischemia group and 58 pmol/L (42-86 pmol/L) in the 45-minute
intestinal ischemia group. Both these groups were increased compared with the sham-operated
group (29 pmol/L; range, 22-57 pmol/L; P <.001 and P <.005, respectively) and the control group (28 pmol/L; range, 26-42 pmol/L; P <.001 and P <.0005, respectively). The median plasma concentration of insulin in the 30-minute
intestinal ischemia group was 4230 pmol/L (range, 1360-5770 pmol/L), which was increased
compared with both the control group (950 pmol/L; range, 550-1510 pmol/L; P <.005) and the sham-operated group (720 pmol/L; range, 280-4180 pmol/L; P<.005). There were no differences between any of the other groups either for glucose,
insulin, or amylin. Conclusions. Plasma amylin concentration is related to the severity of intestinal ischemic injury.
(Surgery 2001;129:730-5.)
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Article info
Publication history
Accepted:
December 22,
2000
Footnotes
*Supported by the Maurice and Phyllis Paykel Trust, the University of Auckland Staff Research Fund, the Auckland Medical Research Foundation, and the Health Research Council of New Zealand.
**Reprint requests: Dr Anthony R. J. Phillips, Level 4 Thomas Building, School of Biological Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.
★Surgery 2001;129:730-5
Identification
Copyright
© 2001 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
