Abstract
Background. High levels of glucose have previously been shown to inhibit endothelial cell migration
and increase secretion of the von Willebrand factor (vWF), a marker of endothelial
cell damage. This study investigates whether thiamine, an important coenzyme in intracellular
glucose metabolism, improves endothelial cell migration and decreases von Willebrand
factor secretion under hyperglycemic conditions. Methods. Bovine aortic endothelial cells (BAECs) were grown under physiological glucose (5.5
mmol/L) and hyperglycemic (13.8 mmol/L and 27.7 mmol/L) conditions with or without
thiamine (200 μmol/L) supplementation. Endothelial cell migration was investigated
in monolayers of BAECs that were wounded by scraping. The distance of migration, the
number of migrating cells, and the surface area covered by the migrating cells were
measured. Secretion of vWF by BAECs under physiological glucose and high glucose conditions
with or without thiamine (200 μmol/L) supplementation was studied with enzyme-linked
immunosorbent assay. Results. Under hyperglycemic conditions, there was a significant decrease in the number of
endothelial cells and an increase in the secretion of vWF (P <.001). Thiamine treatment limited this inhibitory effect of elevated glucose levels
on BAECs. Glucose (27.7 mmol/L) significantly decreased the migration distance of
BAECs into the wounded area to 4.0 ± 1.4 cm, as compared with 6.2 ± 0.3 cm in the
control. Thiamine supplementation restored the migration distance by BAECs (6.94 ±
0.7 cm) and the wound surface area covered (47.7 ± 5.6 cm2)(P <.001). Conclusions. Hyperglycemia activates BAECs and promotes secretion of vWF, a marker of endothelial
cell damage. Thiamine inhibits this endothelial cell activation and the effects of
hyperglycemia on endothelial cell migration. This beneficial effect of thiamine limiting
endothelial cell dysfunction is possibly through the diversion of glucose flux from
anaerobic to aerobic pathways. The data from this study lead to the speculation that
thiamine intake may mitigate or delay vascular complications of diabetes. (Surgery
2001;130:851-8.)
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Article info
Publication history
Accepted:
May 5,
2001
Footnotes
*Supported by a grant from the Maimonides Research and Development Foundation.
**Reprint requests: Theresa Jacob, PhD, Director, Molecular Biology Research Labs, Division of Vascular Surgery, Maimonides Medical Center, 4802 10th Ave, Brooklyn, NY 11219.
Identification
Copyright
© 2001 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
