Abstract
Background. We aimed to use microdialysis to assess, for the first time, the internal milieu
of pancreatic islet grafts. Methods. One month after transplantation, microdialysis probes were inserted into syngeneic
rat islet transplants (500-700 islets) placed beneath the renal capsule of nondiabetic
or diabetic recipients. The number of grafted islets was purposely chosen not to cure
the diabetic recipients. Results. During an intravenous glucose challenge, insulin concentrations increased in parallel
in serum and in the graft interstitium of nondiabetic animals suggesting the existence
of a functionally well-established vascularization. Diabetic recipients had both a
lower serum and dialysate insulin concentration than normoglycemic animals. The lactate/pyruvate
ratios were determined in the dialysates as a measure of the degree of anaerobic metabolism
in the islet grafts. Lactate/pyruvate ratios were between 50 and 100 in grafts of
both nondiabetic and diabetic recipients, and they almost doubled in response to the
intravenous glucose challenge in the grafts of nondiabetic recipients. In comparison,
lactate/pyruvate ratios were approximately 12 in the medium of cultured islets incubated
at low glucose (5.6 mmol/L) or high glucose (16.7 mmol/L) concentrations. Conclusions. The microdialysis technique has proven to be a valuable tool for evaluating the internal
environment of islet transplants. Moreover, the high lactate/pyruvate ratio suggests
that islet grafts have an increased anaerobic glucose metabolism. (Surgery 2002;132:487-94.)
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Article info
Publication history
Accepted:
May 5,
2002
Footnotes
*Supported by grants from the Swedish Medical Research Council (72X-109, 12X-11203), the Juvenile Diabetes Research Foundation, the Swedish-American Diabetes Research Program funded by the Juvenile Diabetes Research Foundation and the Wallenberg Foundation, the Swedish Diabetes Association, the Swedish Society of Medicine, the Novo Nordic Fund, Svenska Barndiabetesfonden, Magnus Bergvalls Stiftelse, Familjen Ernfors Fond, Thurings Stiftelse, Lars Hiertas Minne, Harald och Greta Jeanssons Stiftelse, Göran Gustafsson Foundation, and the Swedish Foundation for Strategic Research.
**Reprint requests: Per-Ola Carlsson, MD, PhD, Department of Medical Cell Biology, Biomedical Center, Husargatan 3, Box 571, SE-751 23 Uppsala, Sweden.
Identification
Copyright
© 2002 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
