Background
During small-bowel transplantation, necrosis and apoptosis are involved in the destruction
of intestinal epithelial cells. This study was conducted to assess which mode of cell
death plays a greater role as a trigger of the bacterial translocation (BT) associated
with intestinal transplantation.
Methods
The following experimental groups were studied: sham, Tx (intestinal transplantation),
Tx + poly (ADP-ribose) synthetase (PARS) inhibitor 3-aminobenzamide (3-AB), and Tx + caspase inhibitor Z-VAD-fmk. Histological analysis, caspase-3 activity, DNA fragmentation,
and BT were measured in tissue samples after transplantation.
Results
During intestinal transplantation, apoptosis and necrosis both increased, showing
graft injury and high levels of BT. Rats treated with 3-AB showed histological protection
of the transplanted graft and a tendency toward lower BT despite the existence of
high apoptosis levels. The rats treated with Z-VAD showed histological protection
of the transplanted graft and decreased levels of caspase-3 and DNA fragmentation.
The Tx + Z-VAD group showed the lowest levels of BT in all tissues.
Conclusions
In small intestinal transplantation, both apoptosis and cell necrosis give rise to
histological injury and BT. Apoptosis inhibition and necrosis inhibition treatments
protect intestinal grafts from ischemia/reperfusion injury; Z-VAD supplementation
has a greater effect on BT prevention than does administration of the PARS inhibitor
3-AB.
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Article info
Publication history
Accepted:
June 4,
2004
Barcelona, SpainFootnotes
Supported by FIS 01/1260.
Identification
Copyright
© 2005 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
