Background
Oxidative stress during cold small bowel (SB) storage has not been investigated because
oxygen is depleted rapidly after procurement. We hypothesized that oxidative catabolism
facilitated by a proven amino acid–based (AA) storage solution promotes oxidative
stress; furthermore, there is an important role for antioxidant supplementation during
cold storage.
Methods
SB from Sprague-Dawley rats (n = 6 in each group) were procured according to standardized
procedures involving vascular flush with modified University of Wisconsin solution
and luminal treatment with an AA-based solution proven previously to aid preservation.
SB were assigned randomly to the following antioxidant treatment groups: group 1,
none; group 2, superoxide dismutase/catalase; group 3, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic
acid (Trolox, a water-soluble analogue of vitamin E). Energetics, oxidative stress,
electrophysiology, and histology were assessed over 24 hours at 4°C.
Results
The addition of Trolox in group 3 resulted in a significant reduction in malondialdehyde
levels compared with all other groups throughout 24 hours of cold storage. Tissue
energetics correlated well with reduced oxidative injury; over the first 12 hours,
adenosine triphosphate and total adenylates were superior in tissues treated with
Trolox (group 3) versus AA solution alone (group 1). Functional assessment showed
relatively normal permeability in all groups, however, Trolox-treated tissues showed
significantly higher short-circuit current compared with control group (17.7 vs 5.5
μA/cm2). Histologic integrity was improved in group 3 after 24 hours of cold storage.
Conclusions
Oxidative stress appears to be a determinant in the pathogenesis of mucosal injury
during cold storage. Trolox effectively abrogates storage-related oxidative stress
in SB.
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References
- The current state of intestinal transplantation.Transplantation. 2004; 78: 175-178
- 2003 report of the intestinal transplant registry.Ann Surg. 2005; 241: 607-613
- Advances in small intestine transplantation.Transplantation. 2004; 77: S75-S78
- Clinical intestinal transplantation.Ann Surg. 2001; 234: 404-416
- Small bowel transplantation.in: Morris P.J. Wood W.C. Oxford textbook of surgery. 2nd ed. 1999
- Defining the role of a tailored luminal solution for small bowel preservation.Am J Transplant. 2002; 2: 229-236
- Human small bowel storage.Transplantation. 2003; 76: 709-714
- Ameliorating small bowel injury using a cavitary two-layer preservation method with perfluorocarbon and a nutrient-rich solution.Am J Transplant. 2004; 4: 1421-1428
- Preserving the mucosal barrier during small bowel storage.Transplantation. 2003; 76: 911-917
- The role of mitochondria in ischemia/reperfusion injury.Transplantation. 2002; 73: 493-499
- Impact of vitamin E supplement in standard laboratory animal diet on microvascular manifestation of ischemia/reperfusion injury.Free Radic Biol Med. 1995; 19: 919-926
- Protective effect of a novel antioxidant non-steroidal anti-inflammatory agent (compound IA) on intestinal viability after acute mesenteric ischemia and reperfusion.Eur J Pharmacol. 2003; 465: 275-280
- The beneficial effect of Trolox on sepsis-induced hepatic drug metabolizing dysfunction.Arch Pharm Res. 2004; 27: 232-238
- Oxidative stress and cardiac microvascular structure in ischemia and reperfusion.Microvasc Res. 2002; 64: 265-277
- The water-soluble vitamin E analogue Trolox protects against ischaemia/reperfusion damage in vitro and ex vivo. A comparison with vitamin E.Pharmacol Res. 2002; 45: 435-439
- Addition of a water-soluble alpha-tocopherol analogue to University of Wisconsin solution improves endothelial viability and decreases lung reperfusion injury.J Surg Res. 1999; 86: 145-149
- Enzymatic analysis. Humana Press, Totowa, NJ1993
- Protein measurement with the Folin phenol reagent.J Biol Chem. 1951; 193: 265-275
- Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.Anal Biochem. 1979; 95: 351-358
- The use of monoclorobimane to determine hepatic GSH levels and synthesis.Anal Biochem. 1990; 190: 212-219
- The sequence of development of intestinal tissue injury after strangulation ischemia and reperfusion.Surgery. 1990; 107: 574-580
- Increased permeability occurs in rat ileum following induction of pancolitis.Dig Dis Sci. 1996; 41: 405-411
- Alleviating ischemia-reperfusion injury in small bowel.Am J Transplant. 2004; 4: 728-737
- Nutrient-related issues affecting successful experimental orthotopic small bowel transplantation.Transplantation. 2005; (in press)
- A novel technique of hypothermic luminal perfusion for small bowel preservation.Transplantation. 2003; 76: 71-76
- Role of the gut in multiple organ failure.World J Surg. 1996; 20: 411-417
- Oxygen in biology and biochemistry.in: Storey K.B. Functional metabolism. Wiley-Liss Inc, Hoboken, NJ2004: 319-368
- The effects of alpha-tocopherol on site-specific lipid peroxidation induced by iron in charged micelles.Arch Biochem Biophys. 1988; 260: 153-160
- Trolox protects rat hepatocytes against oxyradical damage and the ischemic rat liver from reperfusion injury.Hepatology. 1991; 13: 575-580
- The glutathione status of cells.Int Rev Cytol. 1978; 54: 109-156
- Cell suicide in health and disease.Sc Am. 1996; (December): 79-87
- Orrenius S. Signalling mechanisms and oxidative stress in apoptosis.Toxicol Lett. 1995; 82/83: 149-153
Article info
Publication history
Accepted:
August 6,
2005
Received in revised form:
August 4,
2005
Received:
March 15,
2005
Footnotes
Supported by operating funds awarded by the Canadian Institutes of Health Research.
Identification
Copyright
© 2006 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
