This paper is only available as a PDF. To read, Please Download here.
Background. Neutrophils have been shown to play a role in ischemia-reperfusion injury, and the
initial interaction of neutrophils with the endothelium is mediated through the selectin
family of adhesion molecules. Thus the purpose of these studies was to determine whether
a P-selectin-IgG chimera was protective in a model of ischemia-reperfusion injury.
Methods. The model used was a rabbit ear model of ischemia-reperfusion. Selectin-IgG chimeras
were given at the time of reperfusion of the tissue, and their efficacy was compared
with an anti-CD18 antibody (MHM23).
Results. The P-selectin-IgG was as protective in this model as an anti-CD18 antibody. The
chimera did not mediate its effect by causing the animals to become neutropenic.
Conclusions. P-selectin plays a role in ischemia-reperfusion injury. This is in agreement with
data from other groups. The fact that the chimera was effective in this model suggests
that carbohydrates or small molecule mimics of carbohydrates would be effective in
this model. Such antiinflammatory agents may have fewer side effects in terms of increased
risk of sepsis.
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to SurgeryAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Leukocyte adhesion deficiency: an inherited defect in Mac-1, LFA-1, and p150, 95 glycoproteins.Ann Rev Med. 1987; 38: 175-194
- Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins.Science. 1989; 243: 1160-1165
- Cloning of a lymphocyte homing receptor reveals a lectin domin.Cell. 1989; 56: 1045-1055
- Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains.Science. 1989; 243: 1165-1172
- Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarly to proteins involved in cell adhesion and inflammation.Cell. 1989; 56: 1033-1044
- Neutrophils roll on E-selectin.J Immunol. 1993; 151: 6338-6346
- Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins.Cell. 1991; 65: 859-873
- Lectin-like adhesion molecule-1 mediates leukocyte rolling in mesenteric venules in vivo.Blood. 1991; 77: 2552-2555
- L-selectin mediates neutrophil rolling in inflamed venules through sialyl Lewis X-dependent and independent recognition pathways.Blood. 1993; 82: 182-191
- Two step model of leukocyte-endothelial cell interaction in inflammation: distinct roles for LECAM-1 and leukocyte beta-2 integrins in vivo.in: Proc Natl Acad Sci USA. 88. 1991: 7538-7542
- Regulation of leukocyte rolling and adhesion to high endothelial venules through the cytoplasmic domain of L-selectin.J Exp Med. 1993; 177: 833-838
- E-selectin supports neutrophil rolling in vitro under conditions of flow.J Clin Invest. 1993; 92: 2719-2730
- Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity.Cell. 1991; 67: 1033-1036
- Role of neutrophils in ischemia-reperfusion induced microvascular injury.Am J Physiol. 1987; 253: H699-H703
- A monoclonal antibody to the adherence promoting leukocyte glycoprotein CD18 reduces organ injury and improves survival from hemorrhagic shock and resuscitation in rabbits.J Clin Invest. 1988; 81: 939-944
- Role of neutrophils in generalized reperfusion injury associated with resuscitation from shock.Surgery. 1989; 106: 509-516
- Inhibition of leukocyte adherence by anti-CD18 monoclonal antibody attenuates reperfusion injury in the rabbit ear.in: Proc Natl Acad Sci USA. 81. 1990: 939-944
- Reduction of experimental canine myocardial reperfusion injury by a monoclonal antibody (anti-Mo-1, anti-CD11b) that inhibits leukocyte adhesion.J Clin Invest. 1988; 81: 624-629
- Inhibition of CD18 dependent neutrophil adherence reduces organ injury after hemorrhagic shock in primates.Surgery. 1990; 108: 205-212
- A CD18 monoclonal antibody increases the incidence and severity of subcutaneous abscess formation after high-dose Staphylococcus aureus injection in rabbits.Surgery. 1989; 110: 213-219
- The three members of the selectin receptor family recognize a common carbohydrate epitope, the sialyl Lewis X oligosaccharide.J Cell Biol. 1992; 117: 895-902
- P- and E-selectin use common sites for carbohydrate recognition and adhesion.J Cell Biol. 1993; 120: 1227-1235
- Designing CD4 immunoadhesins for AIDS therapy.Nature. 1989; 337: 525-531
- The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies.J Immunol. 1985; 134: 3272-3280
- A human lymphocyte-associated antigen involved in cell-mediated lympholysis.Eur J Immunol. 1993; 13: 202-208
- Anti P-selectin monoclonal antibody attenuates reperfusion injury to the rabbit ear.J Clin Invest. 1993; 92: 2042-2047
- Monoclonal antibodies to leukocyte and endothelial adhesion molecules attenuate ischemia-reperfusion injury.Behring Inst Mitt. 1993; 92: 229
- In vivo neutralization of P-selectin protects feline heart and endothelium in myocardial ischemia and reperfusion injury.J Clin Invest. 1993; 91: 2620-2629
- Role of endothelial-leukocyte adhesion molecule 1 (ELAM-1) in neutrophil-mediated lung injury in rats.J Clin Invest. 1991; 88: 1396-1406
- Neutrophil-dependent acute lung injury—requirement for P-selectin (GMP-140).J Clin Invest. 1992; 90: 1600-1607
- Neutrophil influx into an inflammatory site inhibited by a soluble homing receptor-IgG chimaera.Nature. 1991; 349: 164-167
- Protective effects of selectin chimeras in neutrophil mediated lung injury.J Immunol. 1993; 151: 6410-6417
- Protective effects of oligosaccharides in P-selectin-dependent lung injury.Nature. 1993; 364: 149-151
- Cell surface P- and E-selectin support shear-dependent rolling of gamma/delta T cells.J Immunol. 1994; 153: 3917-3928
- Pulmonary microvascular injury after intestinal ischemia-reperfusion: role of P-selectin.J Appl Physiol. 1993; 75: 2529-2534
- Ischemia reperfusion injury in the rabbit ear is reduced by both immediate and delayed CD18 leukocyte adherence blockade.J Immunol. 1994; 153: 2234-2238
Article info
Publication history
Accepted:
September 26,
1994
Identification
Copyright
© 1995 Mosby-Year Book, Inc. Published by Elsevier Inc.
