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Background. Several growth factors have been shown to enhance incisional wound healing in different
models, but the models have been difficult to compare, and the effects under ischemic
conditions are unknown.
Methods. An ischemic model was developed by selective interruption of the rabbit ear circulation
and placement of dorsal incisions. The model was defined during a 28-day period by
use of serial blood gas analysis, breaking strength measurement, and histologic analysis.
In a separate experiment, incisions were treated with topical growth factors with
the contralateral ear serving as paired controls, and the wounds were evaluated similarly.
Results. The ischemic ears were more hypoxic than controls through day 14 after wounding (48.5
versus 41 mm Hg, p<0.03), and healing was impaired through day 28 (3.21 versus 1.90
newtons, p≤0.001). Transforming growth factor-β (1 μg) and Kaposi's fibroblast growth
factor (20 μg) increased breaking strengths under both normal (3.03 versus 2.41 and
2.83 versus 2.47 newtons, respectively; p<0.05) and ischemic conditions (1.40 versus
1.11 and 1.56 versus 1.23 newtons, respectively; p<0.05). Platelet-derived growth
factor-BB (10 μg) was effective only under normal conditions (2.67 versus 2.15 newtons,
p<0.02), whereas basic fibroblast growth factor (20 μg) was ineffective under both
conditions.
Conclusions. The rabbit ear incisional model is a reproducible ischemic incisional model allowing
comparison of growth factor effects under ischemic and nonischemic conditions. Transforming
growth factor-β and Kaposi's fibroblast growth factor are both effective under ischemic
conditions, whereas basic fibroblast growth factor and platelet-derived growth factor-BB
are not, suggesting that ischemia is an important parameter affecting growth factor
actions.
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Article info
Publication history
Accepted:
October 25,
1994
Footnotes
*Supported in part by grant GM 41303 from the National Institutes of Health.
Identification
Copyright
© 1995 Mosby-Year Book, Inc. Published by Elsevier Inc.
