The immune reaction of the peritoneum to growing gastrointestinal neoplasms remains unclear. We investigated mobilization of immune cells in peritoneal fluid of gastric and colon cancer, phenotypes and level of activation of recruited cells, and concentration of cellular and peritoneal fluid cytokines.
Peritoneal cells (PCs) were obtained intraoperatively by peritoneal lavage from 18 patients with adenocarcinoma of the stomach and 32 patients with adenocarcinoma of the colon (all were stage T2N0M0) and 52 patients who underwent elective cholecystectomy as control subjects.
The number of PCs harvested from cancer patients was 25 times greater than from control patients (P < .001). In the patients with colon cancer, the percentage of CD68+ macrophages was 1.2 times, of CD14+ monocytes was 2.3 times, and of CD15+ granulocytes was 3 times greater than in control patients (all P < .05). The percentage of HLA DR+ cells exceeded the control values by a factor of 2; and within this population, the percentage of CD3+ HLA DR+ cells exceeded control patients by a factor of 3 (P < .05). The percentage of cytokine-producing cells was greater than in control patients, with values 2 times higher for interleukin (IL)-1, 2.5-times for IL-6, and 6-times for IL-8 (P < .05). The concentration of IL-1 in peritoneal fluid exceeded control values by a factor of 2.2, of IL-6 by a factor of 5.0, of IL-8 by a factor of 3, and of monocyte chemotactic protein–1 by a factor of 2.0 (P < .05). In the patients with gastric cancer, the values for mobilized PC granulocytes were 1.5 times greater than in control patients (P < .05). The frequency rate of cytokine-producing cells remained close to control values. The concentration of peritoneal cytokines did not exceed normal values for IL-1 but was 4 times higher for IL-6 and 2 times higher for IL-8 and monocyte chemotactic protein–1 (all P < .05). When the cancer groups were compared, there was evidently more activated myeloid- and cytokine-producing PC in the patients with colon cancer than in patients with gastric cancer. There was no correlation between the blood and PC phenotype frequency.
Patients with T2N0M0 colon cancer and to lesser extent gastric cancer evoke a slight but measurable mobilization and activation of PCs.
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- Characteristics of peripheral and peritoneal lymphocytes from continuous ambulatory peritoneal dialysis patients.Perit Dial Int. 1993; 13: S273-S277
- Human resident peritoneal macrophages: phenotype and biology.Br J Haematol. 1994; 88: 712-722
- Serial cytokine changes in peritoneal effluent and plasma during peritonitis in patients on continuous ambulatory peritoneal dialysis (CAPD).Inflamm Res. 1997; 46: 132-136
- Human peritoneal macrophage and T lymphocyte populations in mild and severe endometriosis.Am J Reprod Immunol. 1995; 34: 179-187
- Gynecology: decrease in interferon gamma production and impairment of T-lymphocyte proliferation in peritoneal fluid of women with endometriosis.Am J Obstet Gynecol. 1996; 175: 1236-1241
- Tumor-associated lympho-monocytes from neoplastic effusions are immunologically defective in comparison with patient autologous PBMCs but are capable of releasing high amounts of various cytokines.Int J Cancer. 1997; 71: 724-731
- Serum and ascitic fluid levels of interleukin-1, interleukin-6, and tumor necrosis factor-alpha in patients with ovarian epithelial cancer.Cancer. 1993; 72: 2433-2440
- Expression of cytokine genes or proteins and signaling molecules in lymphocytes associated with human ovarian carcinoma.Int J Cancer. 1996; 68: 276-284
- Tumor lymphocytes in patients with advanced ovarian cancer: changes during in vitro culture and implications for immunotherapy.Gynecol Oncol. 1997; 65: 391-398
- The characterization of peritoneal and pleural exudate cells from malignant effusions.Jpn J Surg. 1993; 23: 500-503
- Normal human immune peritoneal cells: phenotypic characteristics.Immunobiology. 1989; 180: 80-92
- Normal human immune peritoneal cells: subpopulations and functional characteristics.Scand J Immunol. 1996; 44: 157-163
- Involvement of inflammatory mediators in macrophage antitumor activity.J Leukoc Biol. 1993; 54: 613-626
- Cytokines as positive and negative regulators of tumor promotion and progression.Semin Cancer Biol. 1992; 3: 3-15
- Interstitial pressure gradients in tissue-isolated and subcutaneous tumors: implications for therapy.Cancer Res. 1990; 50: 4478-4484
- The usefulness of CD4/CD8 ratio evaluation in monitoring of ovarian cancer patients.Eur J Gynaecol Oncol. 1995; 16: 54-58
- Generation of lymphokine-activated killer (LAK) cell activity from malignant peritoneal effusions.Life Sci. 1995; 19: 92-98
- Loss of CD45R and a gain of UCHL1 reactivity is a feature of primed T cells.J Immunol. 1988; 140: 2171-2178
- Differential activation requirements for virgin and memory T cells.J Immunol. 1988; 141: 3249-3257
- Migration pathways and immunologic memory among T lymphocytes.Semin Immunol. 1992; 4: 51-58
- Studies of lymphocyte transendothelial migration: analysis of migrated cell phenotypes with regard to CD31 (PECAM-1), CD45RA and CD45RO.Immunology. 1993; 80: 553-560
- FACS analysis of peritoneal lymphocytes in ovarian cancer and control patients.Immunobiology. 1994; 191: 1-8
- Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin.Nature. 1986; 324;: 73-76
- Release of hydrogen peroxide and expression of HLA-DR and transferrin receptors by monocytes and peritoneal macrophages from patients undergoing continuous ambulatory peritoneal dialysis and normal controls.Clin Immunol Immunopathol. 1990; 56: 151-158
- Transferrin receptor (CD71) expression in peritoneal macrophages from fertile and infertile women with and without endometriosis.Am J Reprod Immunol. 1997; 38: 413-417
- Tumor necrosis factor and interleukin 1: cytokines with multiple overlapping biological activities.Lab Invest. 1987; 56: 234-248
- B cell stimulatory factor-2 is involved in the differentiation of cytotoxic T lymphocytes.J Immunol. 1988; 140: 508-512
- IL1 is required for tumor invasiveness and angiogenesis.Proc Natl Acad Sci USA. 2003; 100: 2645-2650
- Alterations in gastric mucin with malignant transformation: novel pathway for mucin synthesis.J Natl Cancer Inst. 1995; 87: 441-446
- Expression of Tn, sialosyl-Tn, and T antigens in human colon cancer.Cancer Res. 1989; 49: 197-204
Published online: October 04, 2006
Accepted: June 27, 2006
Received in revised form: June 22, 2006
Received: April 14, 2005
Supported by Komitet Badan Naukowych (Committee of Scientific Research) grant no. 4S402 128 07.
© 2007 Mosby, Inc. Published by Elsevier Inc. All rights reserved.