Background
The immune reaction of the peritoneum to growing gastrointestinal neoplasms remains
unclear. We investigated mobilization of immune cells in peritoneal fluid of gastric
and colon cancer, phenotypes and level of activation of recruited cells, and concentration
of cellular and peritoneal fluid cytokines.
Methods
Peritoneal cells (PCs) were obtained intraoperatively by peritoneal lavage from 18
patients with adenocarcinoma of the stomach and 32 patients with adenocarcinoma of
the colon (all were stage T2N0M0) and 52 patients who underwent elective cholecystectomy
as control subjects.
Results
The number of PCs harvested from cancer patients was 25 times greater than from control
patients (P < .001). In the patients with colon cancer, the percentage of CD68+ macrophages was 1.2 times, of CD14+ monocytes was 2.3 times, and of CD15+ granulocytes was 3 times greater than in control patients (all P < .05). The percentage of HLA DR+ cells exceeded the control values by a factor of 2; and within this population, the
percentage of CD3+ HLA DR+ cells exceeded control patients by a factor of 3 (P < .05). The percentage of cytokine-producing cells was greater than in control patients,
with values 2 times higher for interleukin (IL)-1, 2.5-times for IL-6, and 6-times
for IL-8 (P < .05). The concentration of IL-1 in peritoneal fluid exceeded control values by
a factor of 2.2, of IL-6 by a factor of 5.0, of IL-8 by a factor of 3, and of monocyte
chemotactic protein–1 by a factor of 2.0 (P < .05). In the patients with gastric cancer, the values for mobilized PC granulocytes
were 1.5 times greater than in control patients (P < .05). The frequency rate of cytokine-producing cells remained close to control
values. The concentration of peritoneal cytokines did not exceed normal values for
IL-1 but was 4 times higher for IL-6 and 2 times higher for IL-8 and monocyte chemotactic
protein–1 (all P < .05). When the cancer groups were compared, there was evidently more activated
myeloid- and cytokine-producing PC in the patients with colon cancer than in patients
with gastric cancer. There was no correlation between the blood and PC phenotype frequency.
Conclusion
Patients with T2N0M0 colon cancer and to lesser extent gastric cancer evoke a slight
but measurable mobilization and activation of PCs.
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Article info
Publication history
Published online: October 04, 2006
Accepted:
June 27,
2006
Received in revised form:
June 22,
2006
Received:
April 14,
2005
Footnotes
Supported by Komitet Badan Naukowych (Committee of Scientific Research) grant no. 4S402 128 07.
Identification
Copyright
© 2007 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
